CC2 LAB (ALT, Na, Ca, Amy, Mg, LDH) Flashcards
Calcium Method (ELITechGroup Manual)
Direct colorimetric complexometric test (Arsenazo III)
Reagent Composition of Calcium
MES Buffer, pH 6.5 - 100 mmol/L
Arsenazo III - 200 umol/L
Standard: (Ref: CALA-0600)
Calcium - 10 mg/dL or 2.5 mmol/L
Calcium Reference Values (ELITechGroup Manual)
Serum/Plamsa:
8.6-10.3 mg/dL
2.15-2.57 mmol/L
Urine:
100-300 mg/24H
2.5-7.5 mmol/24H
6.7-20.0 mg/dL
1.67-5.00 mmol/L
Always interpreted according to the plasmatic protein rates under Calcium Test
Calcemia
Calculation under Calcium Test
A Sample
——————————— x n
A Standard or Calibrator
Serum Volume for Calcium Test (ELITechGroup Manual)
20 microliter
Reagent Volume for Calcium Test (ELITechGroup Manual)
1000 microliter
Incubation time for Calcium Test (ELITechGroup Manual)
1 minute