Analysis of lipids

Question 1

Sample preferred

Answer 1

Serum

Question 2

Standing plasma test

Answer 2

Stick in refrigerator and test for chylomicrons
Cream layer on top-> chylomicrons present
Turbidity without cream layer on top-> latescence due to VLDLs

Question 3

If visual plasma turbidity is present

Answer 3

-TG will be increased because the plasma contains high concentration of a TG rich lipoproteins (chylomicron or VLDL)
-Cholesterol typically increased because plasma contains high conc. of lipoproteins that contain cholesterols

Question 4

If visual plasma turbidity is not present

Answer 4

-Tg may still be increased because plasma contains high concentration of TG rich lipoprotein (chylomicron or VLDL)
-Cholesterol may still be increased cholesterol rich molecules (LDL and HDL ) due not cause visual lipemia

Question 5

NEFAs

Answer 5

Free FA
Produced by breakdown of TG and increase in response to negative energy balance