84. Culture of mycoplasmas. Ovine and caprine pathogenic Mycoplasma species.

Question 1

Culture Of Mycoplasmas?

Answer 1

Culture
• fastidious, horse serum (sterols, fatty acids)
• yeast extract, DNA, lactalbumin hydrolysate, urea, cysteine
• no turbidity
• typical colony morphology (like a nipple)
7-14 days, CO2
• selective isolation: penicillin, tallium acetate
• some species cannot be cultured: M. (E.) suis
M. (E.) ovis
M. (H.) haemocanis
M. (H.) haemofelis

Question 2

Sheep Goat Pathogenic species?

Answer 2

Sheep, goat
• M. capricolum ssp. capripneumoniae: Contagious Caprine
pleuropneumonia
• M. capricolum ssp. capricolum: sheep, goat pneumonia, mastitis,
arthritis, septicaemia
• M. mycoides ssp. capri: goat
• M. agalactiae: sheep, goat pneumonia, contagious agalactia,
arthritis
• M. conjunctivae: sheep, goat keratoconjunctivitis
• M. ovipneumoniae: sheep pneumonia, keratoconjunctivitis
• M. (E.) ovis: sheep anaemia (eperythrozoonosis) worldwide