39. Genus Erysipelothrix.

Question 1

Habitat?

Answer 1

ERYSIPELOTHRIX Erysipelothrix rhusiopathiae E. tonsillarum

1. Habitat: • alimentary tract (swine tonsils), mud, slime of fishes, water

Question 2

Morphology?

Answer 2

Morphology:

• 1-3 µm thin rods (S type), chains, filaments (R type)

Question 3

Staining?

Answer 3

Staining: • Gram-positive

Question 4

Culture?

Answer 4

Culture:

• nutrient agar,
• replication 5-42 oC, pH 6.7-9.2
• blood agar: some strains α haemolysis
• S-R colonies
• agar
• broth:
• S: uniform turbidity
• R: sediment
• selective culture:
• NaN3 + crystal violet
• neomycin

Question 5

Biochemistry?

Answer 5

Biochemistry:

• catalase -,
• oxidase -,
• fermentative,
• extra cellular enzymes
• neuraminidase: binding to endothelial cells
• hyaluronidase: spreading

Question 6

Antigens?

Answer 6

Antigens:

• two common protein antigens
• protection
• type specific polysaccharide haptens
• 1-26: diagnostics

Question 7

Resistance?

Answer 7

Resistance:

• good, halotolerance,

soil

Question 8

Pathogenecity?

Answer 8

Pathogenicity:

• facultative pathogenic
• swine: erysipelas
• sheep: wound infection
• birds: septicaemia
• humans: wound infection (erysipeloid)

Question 9

E.tonsillarium?

Answer 9

E. tonsillarum:

• no biochemical difference
• serotype 7 and further 6 serotypes
• present on tonsils of swine,
• endocarditis of dogs