77. Genus Leptospira (culture, typing, subtyping). Important pathogenic Leptospira serotypes.

Question 1

Leptospira Culture?

Answer 1

Culture:

• aerobic, 28-30 oC
• nutrients (free fatty acids, inorganic N, vitamins),
• Korthof-medium
• EMJH medium, other media
• animal trial,
• embryonated egg

Question 2

Leptospira Biochemistry?

Answer 2

Biochemistry:

• demands • free fatty acids, • inorganic N, • small amount of end products, • fructose-6-PO4

Question 3

Resistance?

Answer 3

Resistance: • low

Question 4

Groups?

Answer 4

Groups • complex taxonomy • 21 species • pathogen: 9 species • L. interrogans • L. borgpeterseni • L. kirschneri • saprophyte: 7 species • intermediate: 5 species • serovariants (serovar) • agents of the diseases! • serogroups • related serovars

Question 5

Maintance and incidental hosts?

Answer 5

Question 6

Pathogenecity?

Answer 6