41. Genus Mycobacterium (habitat, morphology, staining, culture). Facultative pathogenic and saprophytic Mycobacterium species.

Question 1

Habitat?

Answer 1

MYCOBACTERIUM
1. Habitat:
• infected host
• obligate pathogens
• environment
• soil, water, plants
• facultative pathogens
• saprophytes

Question 2

Morphology?

Answer 2

2. Morphology:
   • different morphology
   • rods
   • coccoid
   • branching filaments
   • cell structure
   • cell wall
   • mycolic acids, lipids, waxes, surface proteins: virulence
   factors
   • species differences

Question 3

Staining?

Answer 3

Question 4

Culture?

Answer 4

4. Culture:
   • obligate aerobic
   • broth:
   • Henley, Sauton (cord formation)
   • solid:
   • egg (Kertai, Dorset, Petragnani),
   • serum agar,
   • glycerine potato
   • selective:
   • Petragnani (malachite green),
   • inoculation after acid or alkaline treatment,
   • M. avium subsp. paratuberculosis needs mycobactin
   • animal trial (guinea pig, rabbit, pigeon)
   • incubation time, growth characteristics (species differences) 1

Question 5

Culture differences?

Answer 5

Question 6

Facultative pathogenic and saprophytic Mycobacterium species.

Answer 6