75.Genus Brachyspira.

Question 1

Brachyspira habitat?

Answer 1

Habitat: • swine large intestine

Question 2

Brachyspira Morphology?

Answer 2

Morphology: • 7-9 µm helical rods

Question 3

Brachyspira staining?

Answer 3

Staining: • fuchsin, Giemsa, • unstained

Question 4

Brachyspira Culture?

Answer 4

Culture: • obligate anaerobic, • fastidious

Question 5

Brachyspira species?

Answer 5

Species

• B. hyodysenteriae
• B. pilosicoli
• B. intermedia
• B. innocens
• B. alvinipulli

Question 6

Brachyspira Hyodysenteriae?

Answer 6

B. hyodysenteriae:

1. Habitat • pig: gut • mouse, rat, dog (temporarly)
2. Morphology, staining • can be stained • unstained: movement
3. Culture: • fastidious, • selective isolation • spectinomycin – polymyxin - vancomycin • β haemolysis (haemolysin is a virulence factor)
4. Antigens: • 11 serogroups
5. Resistance: • low • faeces: 1 week – 2 months 6. Pathogenicity: • facultative pathogenic, • swine dysentery

Question 7

• B. pilosicoli • B. intermedia • B. innocens • B. alvinipulli ?

Answer 7

B. innocens: • saprophyte, • weak haemolysis, • biochemical differences

B. pilosicoli: • weak haemolysis, • swine intestinal spirochaetosis • avian intestinal spirochaetosis

B. intermedia: • weak haemolysis, • swine diarrhoea B. alvinipulli • poultry intestinal spirochaetosis