69. Genera Pseudomonas and Aeromonas.

Question 1

Pseudomonas Habitat,morphology and staining?

Answer 1

PSEUDOMONAS

1. Habitat: • mucous membranes • soil, water, plants, environment
2. Morphology: • 1.5-5 µm rod, • flagella
3. Staining: • Gram-negative

Question 2

Pseudomonas Culture?

Answer 2

Culture:

• not fastidious • nutrient agar • enterobacterium selective media (low selective capacity) • obligate aerobic, • pigment production

Question 3

Pseudomonas pathogenicity?

Answer 3

Pathogenicity:

• saprophytes – plant pathogens – animal pathogens

Question 4

Species Pseudomonas?

Answer 4

Species

• Pseudomonas aeruginosa
• Pseudomonas anguilliseptica

Question 5

P.aeruginosa Habitat Morphology?

Answer 5

P. aeruginosa

1. Habitat: • environment, water, sewage, gut
2. Morphology: • 1 polar flagellum, polar fimbria

Question 6

P.aeruginosa Culture?

Answer 6

3. Culture:

• simple, 5-42 oC,
• β haemolysis,
• odour
• pigments:
• piocianin
• fluorescein

Question 7

P.aeruginosa Biochemistry?

Answer 7

Biochemistry:
• catalase +, oxidase +
• virulence factors
• toxin (exotoxin A)
• extra cellular enzymes (protease, phospholipase C)
• production of exo-polysaccharide (biofilm formation)

Question 8

P.aeruginosa Antigens?

Answer 8

. Antigens: • O antigen • 27 O antigens, several components • H antigens • fimbria • phage typing, • pyocin typing

Question 9

P.aeruginosa Resistance?

Answer 9

Resistance: • resistance against • quaterner NH4 salts, • amphoteric disinfectants • susceptibility • dehydration, • heat, • most disinfectants

Question 10

P.aeruginosa Pathogenicity?

Answer 10

. Pathogenicity: • facultative pathogenic, • local suppuration, • cattle, mare: • mastitis, metritis, • sheep: • dermatitis • dog: • otitis externa, • fur animals: • septicaemia • human • middle ear infection, secondary agent, immunocompromised • burn, nosocomial (hospital) infections

Question 11

P.anguillseptica Biochemistry Pathogenicity?

Answer 11

P. anguilliseptica 1. Biochemistry: • inactive 2. Pathogenicity: • salmon, eel: • septicaemia, • keratitis, • haemorrhages

Question 12

Aeromonas Habitat morphology staining culture?

Answer 12

AEROMONAS

1. Habitat: • water, sewage, soil, environment • gut (mammals, birds, cold blooded animals)
2. Morphology: • 1-4 µm rods, • generally flagellated 3. Staining: • Gram-negative
3. Culture: • simple, aerobic, facultative anaerobic

Question 13

Aeromonas Biochemistry?

Answer 13

. Biochemistry:

• catalase +, • oxidase + • virulence factors • toxins (enterotoxins, haemolysin) • extra cellular enzymes

Question 14

Aeromonas species?

Answer 14

Groups, species

• „mesophilic aeromonas” species:
• e.g. A. hydrophila • „psychrofilic aeromonas” species:
• e. g. A. salmonicida

Question 15

A. hydrophillia Morphology?

Answer 15

A. hydrophila

1. Morphology: • 1-4 µm rods, • flagella

Question 16

A.hydrophillia Culture?

Answer 16

Culture: • simple, • haemolysis, • optimal temperature 28 oC

Question 17

A.hydrophillia Biochemistry?

Answer 17

3. Biochemistry: • sometimes gas is produced • resembles E. coli

Question 18

A.hydrophillia Pathogenicity?

Answer 18

Pathogenicity:

• facultative pathogenic, • fishes, amphibians, reptiles • septicaemia, inflammation of the swimming bladder • farm animals, humans • asymptomatoic colonisation • food borne infection • sometimes wound infection, local suppuration • septicaemia in immunocompromised persons

Question 19

A.salmonicida Morphology,culture and pathogenicity?

Answer 19

A. salmonicida

1. Morphology: • small coccobacillus, • no flagella
2. Culture: • optimal temperature: 20 oC, • dark brown pigment
3. Pathogenicity: • facultative pathogenic • fish (Salmonidae): furunculosis