35. Genus Staphylococcus (habitat, morphology, culture). Characterisation of coagulase positive Staphylococcus species.

Question 1

Staphyloccus Habitat?

Answer 1

STAPHYLOCOCCUS

1. Habitat:

• skin,
• mucous membrane,
• food, feed, plant,
• soil,
• water

Question 2

Morphology?

Answer 2

Morphology:

• coccus, clusters, bunches of grape

Question 3

Staining?

Answer 3

Staining • Gram-positive

Question 4

Culture?

Answer 4

Culture:

• simple:
• nutrient agar, nutrient broth
• pigment production
• golden yellow, white
• carotenoid
• haemolysis
• β haemolysis (in some species double haemolysis)
• no haemolysis
• selective culture:
• 10% NaCl – mannitol – phenol red agar,
• Na-tellurite – glycine – pyruvic acid – egg (Baird-Parker agar)

Question 5

Coagulase positive species?

Answer 5

• Coagulase positive species:

•S. aureus susp. aureus

• biotypes, different hosts
• cattle, swine, horse, sheep, dog, poultry etc.
• MRSA: methicillin resistant S. aureus: human, dog, horse, pig
• S. aureus subsp. anaerobius • sheep: Morel-disease •S. pseudointermedius • dog, cat: dermatitis, otitis externa
• S. intermedius • dog, horse, bird