67.Genus Brucella (antigens, resistance, typing, subtyping). Characterisation of S-type Brucella species.

Question 1

S colonies:

Answer 1

S colonies: B. melitensis, B. abortus, B. suis

Question 2

Antigens?

Answer 2

Antigens: • lipopolysaccharide, peptides, lipids • close antigen relationship • „S colony type”: • B. melitensis - B. abortus - B. suis cross reaction • A and M polysaccharides: B. melitensis - B. abortus - B. suis • „R colony type”: • B. ovis - B. canis cross reaction

Question 3

Resistance?

Answer 3

Resistance:

• medium
• highly susceptible to acidic pH
• inctivated in fermented dairy products and meat
• 30-day-long survival in non-fermented dairy products
• 30-day-long survival in bedding

Question 4

Species

Answer 4

species:

• phage susceptibility (Tbilisi phage)
• oxidative metabolism,
• natural host
• genome structure

Question 5

Biotype

Answer 5

biotype:

• CO2 demand,
• H2S,
• fuchsin/thionin tolerance,
• agglutination (A-M-R)

Question 6

Pathogenicity

Answer 6

Pathogenicity: • species, hosts

• B. melitensis: goat, sheep, cattle, human

• B. abortus: cattle, sheep, human
• B. suis: swine (biotypes 1-3), hare (biotype 2), reindeer (biotype 4), rodents (biotype 5)
• B. pinnipedialis: seal, walrus

• B. ceti: whale, dolphin

B.ovis sheep

• B. canis: dog,

• B. neotomae: desert rat

• B. microti: vole

Question 7

Pathogenicity 2?

Answer 7

Pathogenicity: • facultative intra cellular bacterium • survives within macrophages • diseases • generalised diseases • acute • abortion • epididymitis, orchitis • subacute • arthritis, bursitis, tenosynovitis