68. Genus Burkholderia.

Question 1

Burkholderia habitat?

Answer 1

Habitat: • mucous membranes •

B. mallei • soil, water, plants •

B. pseudomallei • plant pathogens and saprophytes

Question 2

Burkholderia Morphology?

Answer 2

Morphology: • 1.5-5 µm rod • flagella (except B. mallei)

Question 3

Burkholderia Staining?

Answer 3

Staining: • Gram-negative

Question 4

Culture Burkholderia?

Answer 4

Culture:

• not fastidious,
• obligate aerobic,
• pigment production (exc. B. mallei)

Question 5

Species Burkholderia?

Answer 5

Species: • B. mallei • B. pseudomallei • close relationship

Question 6

Burkholderia mallei Habitat Morphology and staining?

Answer 6

Burkholderia mallei

1. Habitat: • infected hosts
2. Morphology: • 1.5-5 µm rod • no flagella
3. Staining: • Gram-negative • irregular staining

Question 7

Burkholderia mallei Culture?

Answer 7

Culture:

• glycerol supports growth
• no pigment production

Question 8

Biochemistry and antigens Burkholderia Mallei?

Answer 8

5. Biochemistry: • catalase +, • oxidase +, • oxidative

6. Antigens: • mallein (PPD) • cell wall extract • glycoprotein

Question 9

Burkholderia Resistance and Pathogenecity?

Answer 9

7. Resistance: • weak • dies if dehydrated in the environment • survives in water for several weeks

8. Pathogenicity: • malleus/glanders (horse, donkey), • cat, dog • human

Question 10

Burkholderia pseaudomallei?

Answer 10

Burkholderia pseudomallei

1. Habitat: • soil, water, mud • tropics, subtropics
2. Morphology: • 1.5-5 µm rod • flagella

Question 11

Burkholderia Pseudomallei?

Answer 11

3. Culture: • simple • nutrient agar • glycerol agar: large colonies, • broth: layer • pigmen

4. Pathogenicity: • melioidosis • swine, cattle, sheep, goat, dog, cat, human