36. Genus Staphylococcus (virulence factors, grouping). Characterisation of coagulase negative Staphylococcus species, genus Micrococcus.

Question 1

Biochemistry?

Answer 1

5. Biochemistry:
   • catalase: +, oxidase: -, fermentative
   • extracellular enzymes:
   • coagulase,
   • fibrinolysin,
   • hyaluronidase…
   • toxins:
   • haemolysins
   • leucocidins
   • enterotoxins, dermotoxin (exfoliative toxin) toxic shock
   syndrome toxins (TSST)

Question 2

Antigens, Resistance and pathogenecity?

Answer 2

6. Antigens:
• complex (surface proteins, polysaccharides)
• protein-A
7. Resistance:
• good
8. Pathogenicity:
• local suppuration,
• abscesses,
• arthritis,
• mastitis,
• metritis,
• dermatitis
• septicaemia

Question 3

Species, pathogenecity?

Answer 3

Question 4

Coagulase negative species?

Answer 4

coagulase negatíve species:
• S. epidermidis
• wound infection (horse, dog)
• S. haemolyticus
• milk
• S. hyicus:
• a part of the strains is coagulase positive
• porcine exudative epidermitis
• S. gallinarum
• dermatitis
• S. equorum
• dermatitis
• S. felis
• dermatitis

Question 5

Micrococcus habitat morphology Biochemistry and pathogenecity?

Answer 5

MICROCOCCUS

1. Habitat:

• environment, mucous membrane, food, skin

2. Morphology:

• coccus, clusters (grape)

3. Biochemistry:

• decomposition of glucose is aerobic or missing

4. Pathogenicity:

• saprophyte
• to be differentiated from Staphylococci