56. Genus Fusobacterium.

Question 1

Fusobacterium Morphology and Staining?

Answer 1

FUSOBACTERIUM

1. Morphology: • polymorph • chain, filaments • generally no flagella
2. Staining: • Gram-negative • irregular staining

Question 2

Culture ?

Answer 2

3. Culture:

• fastidious
• blood agar
• protein is needed
• yeast extract
• obligate anaerobic,
• CO2
• haemolysis
• selective culture:
• crystal violet
• brillant green
• vancomycin

Question 3

Biochemistry, Resistance and Pathogenecity?

Answer 3

4. Biochemistry:

• carbohydrates are generally not utilised • gelatine, casein are decomposed • extracellular enzymes (lipase, DN-ase, hyaluronidase)

5. Resistance: • low
6. Pathogenicity: • facultative pathogenic, • anaerobic wounds, abscesses, gangrene

Question 4

Species 1?

Answer 4

Question 5

Species 2?

Answer 5

7. Species
   F. necrophorum subsp. necrophorum:
   • calf, piglet, rabbit:
   • necrobacillosis, calf diphtheria, bovine liver abscess
   • sheep:
   • involved in the pathogenesis of foot rot
   • cattle
   • mastitis
   • dog, cat
   • gingivitis, peritonitis
   • wound infections, abscesses

Question 6

Species 3?

Answer 6

F. nucleatum:

• mainly human, dog, cat

• abscess, • mastitis, metritis, • peritonitis, • wound infection