34. Genus Clostridium (resistance, pathogenicity, grouping). Characterisation of pathogenic Clostridium species (except Clostridium species causing gas gangrene diseases).

Question 1

Biochemistry, Antigens and Resistance?

Answer 1

5. Biochemistry: catalase −, oxidase −, OF: fermentaƟve •active metabolism, carbohydrates are fermented
   (exc. C. tetani, C. histolyticum) •proteolytic: C. tetani, C. histolyticum, C. botulinum
   •exotoxins (toxin, toxoid or anatoxin, antitoxin)
6. Antigens: complex, close relationship
7. Resistance: •Vegetative (like Gr+) •Spore (in dry specimens: for years; C. botulinum: 3-4 h
   boiling (surv.)
   C. perfr. spore: 5-10 min boiling, 8% formalin: 2 hs

Question 2

Enteroxaemia and Intoxications?

Answer 2

enterotoxemic diseases

• necrotic enteritis of chicken: C. perfringens A/C
• lamb dysentery: C. perfringens B
• necrotic enteritis of pigs, struck of sheep: C. perfringens C
• pulpy kidney disease of sheep: C. perfringens D
• ulcerative enteritis of chicken: C. colinum
intoxications

• tetanus: C. tetani
• botulism: C. botulinum

Question 3

C.tetani?

Answer 3

Question 4

C.botulinium?

Answer 4

Answer 5