61. Genera Haemophilus, Histophilus and Avibacterium.

Question 1

Haemophillus habitat?

Answer 1

Habitat: • mucous membranes of humans and animals (respiratory, genital)

Question 2

Haemophillus Morphology?

Answer 2

Morphology: • 0.5-2 µm coccoid rods, filaments, (fimbria)

Question 3

Haemophillus Staining?

Answer 3

Staining: • Gram-negative

Question 4

Haemophillus Culture?

Answer 4

Culture: • fastidious • chocolate agar • additives • haem (X-factor): • blood • NAD (V-factor): • satellitism, NAD, fresh yeast extract, • some species need 10-15 % CO2

Question 5

Haemophillus Biochemistry Antigens Resistance and pathogenecity?

Answer 5

5. Biochemistry: • examination is difficult
6. Antigens: • cell wall antigens • capsule antigens
7. Resistance: weak
8. Pathogenicity: facultative pathogenic

Question 6

Haemophillus Species?

Answer 6

Species:

• H. influenzae: • human upper respiratory ways, • meningitis
• H. parasuis: • V-factor demand, • 15 serotypes • porcine polyserositis (Glässer’s disease)

Question 7

Histophillus Habitat and subspecies?

Answer 7

HISTOPHILUS

Histophilus somni (Haemophilus somnus, Histophilus ovis, Haemophilus agni):

1. Habitat: • urogenital mucous membranes, respiratory way

Question 8

HISTOPHILLUS staining Morpholgy?

Answer 8

Staining, morphology: • Gram-negative, short rods

Question 9

Histophillus Culture?

Answer 9

Culture:

• fastidious
• X, V factor not needed,
• CO2 -demand

Answer 10

4. Pathogenicity:
   • facultative pathogenic
   • cattle
   • calf pneumonia,
   • septicaemia,
   • thromboembolic meningoencephalitis (TEME),
   • abortion
   • mastitis
   • sheep:
   • epididymitis-orchitis
   • abortion
   • mastitis
   • lamb septicaemia

Question 11

Avibacterium Habitat morphology Staining and culture?

Answer 11

AVIBACTERIUM

1. Habitat • respiratory mucous membrane
2. Morphology • 0,5-2 µm coccoid rods, filaments
3. Staining • Gram-negative
4. Culture • fastidious • V faktor-demand is variable • some species need CO2

Question 12

Avibacterium Species?

Answer 12

Species
• Avibacterium (Haemophilus) paragallinarum:
• CO2 and V-factor are needed
• 3 serogroups, 9 subgroups
• infectious coryza of hens
• A. gallinarum
• V-factor is not needed
• mild respiratory disease in hens
• A. avium
• V-factor is needed
• saprophyte, sometimes mild respiratory disease in hens