64. Genus Bordetella.

Question 1

Bordetella Habitat, Morphology, staining and culture?

Answer 1

BORDETELLA

1. Habitat: • respiratory mucous membrane (mammals, birds)
2. Morphology: • 2-3 µm rods
3. Staining: • Gram-negative
4. Culture: • obligate aerobic • generally simple

Question 2

Biochemistry and species?

Answer 2

5. Biochemistry: • catalase +, • oxidase +, • cannot utilise carbohydrates • toxins are produced

6. Species

• B. pertussis: human pertussis
• B. parapertussis: human parapertussis, ovine pneumonia
• B. bronchiseptica

B. avium

Question 3

B. bronchiseptica: Habitat and Morphology?

Answer 3

B. bronchiseptica:

1. Habitat: • mucous membrane, respiratory (swine, dog, cat, horse, rabbit)
2. Morphology: • rods • capsule • flagella • fimbria 3. Staining • Gram-negative

Question 4

B.bronchiseptica Culture?

Answer 4

4. Culture: • simple, • growth on media containing only citrate • selective isolation: • crystal violet, bile salts (MacConkey-agar), penicillin, nitrofurantoin

Question 5

B.brochiseptica Biochemistry and anitigens?

Answer 5

5. Biochemistry: • cytotoxins are produced • dermonecrotoxin: osteoblasts are damaged • tracheatoxin: ciliated epithelial cells are damaged • adenylate cyclase haemolysin: phagocytosis is inhibited

6. Antigens: • several O, K, H and fimbria antigens

Question 6

B.bronchiseptica Resistance and pathogenicity?

Answer 6

7. Resistance: • medium

8. Pathogenicity: • facultative pathogenic, • swine • atrophic rhinitis (with P. multocida), • rabbit • pneumonia • dog • kennel cough • complication to distemper, • cat • bronchitis, bronchopneumonia • sneezing disease

Question 7

B. avium habitat,morphology, culture and biochemistry?

Answer 7

B. avium:

1. Habitat: • poultry respiratory tract
2. Morphology: • capsule, flagella, fimbria
3. Culture: • simple
4. Biochemistry: • cytotoxins • dermonecrotoxin • tracheatoxin

Question 8

Pathogenecity B.avium?

Answer 8

5. Pathogenicity: • avian bordetellosis (turkey coryza), • turkey, • chicken