71. Genus Campylobacter.

Question 1

Habitat?

Answer 1

CAMPYLOBACTER

1. Habitat: • mucous membrane (genitals, oral cavity, intestine), • food

Question 2

Morphology?

Answer 2

Morphology:
• 2-5 µm curved rods,
• S- forms, spiral filaments,
• flagella

Question 3

Staining?

Answer 3

Staining: • Gram-negative

Question 4

Culture?

Answer 4

Culture: • fastidious • yeast extract, organic acids • optimal temperature: • 37 oC (C. fetus) • 42 oC (thermophiles, C. jejuni, C. coli) • microaerophilic, • 6% O2 , 10% CO2 , 84% N2 • selective culture: • vancomycin - polymyxin – trimethoprim - cycloheximide • can be cultured in semisolid media

Question 5

Biochemistry?

Answer 5

Biochemistry: • catalase +/-, • oxidase +, • no carbohydrate utilisation, • biochemical characteristics: • differentiation of species, subspecies

Question 6

Antigens and Resistance?

Answer 6

6. Antigens: • cell wall antigens • C. fetus: O1 and O2 • C. jejuni/C. coli: 66 serogroups 7. Resistance: • weak • susceptible to oxygen

Question 7

Pathogenecity secies?

Answer 7

Pathogenicity, species: • facultative pathogens: catalase + • C. fetus subsp. fetus: • C. fetus subsp. venerealis: • C. jejuni/C. coli: • C. hepaticus • C. lari: • C. ureolyticus • saprophytes: catalase - • C. mucosalis, C. hyointestinalis • C. sputorum

Question 8

Species 1?

Answer 8

Species • C. fetus subsp. fetus: • sheep (cattle) abortion • mammals, birds enteritis • C. fetus subsp. venerealis: • cattle abortion, infertility • C. jejuni/C. coli: • present in the gut • sheep, goat (cattle): abortion, mastitis • dog, cat, poultry, rabbit: enteritis

Question 9

Species 2?

Answer 9

Species • C. hepaticus • campylobacter-hepatitis poultry • C. lari: • wild living birds gut, • pathogenicity like C. jejuni/C. Coli • C. ureolyticus • mares: vaginitis, metritis • C. mucosalis, C. hyointestinalis • swine intestine, saprophytes • C. sputorum: • mucous membranes, saprophytes