1st powerpoint Flashcards
When you fixate a specimen, you have to first remove what?
degradative enzymes, at least neutralize them
To fixate a specimen to look at it under a light microscope, you would utilize what solution?
formalin (formaldehyde)
To fixate a specimen to look at it under a electron microscope, you would utilize what solution?
osmium tetroxide
or
glutaraldehyde
What are the two most common stains?
Hematoxilin and Eosin
What chrage does hematoxilin have?
its a basic dye
What charge does Eosin have?
its an acidic dye; +
What structures will hematoxilin stain?
it will stain acidic structures; RER, nucleoli, heterochromatin
What structures will be stained with eosin?
basic structures; mitochondria,
What color does hematoxilin stain?
blue
what color does eosin stain?
red
What are frozen sections used for?
to examine tissue rapidly
When you stain, what does the staining do to observe a structure(s)?
it disrupts the light beam in order to view a structure
In a cell, using eosin and hematixilin, what organelle will stain blue and what will stain red?
hematoxilin stains nuclei blue
eosin stains cytoplasm red
What is PAS used for?
for staining glycoproteins
What color does PAS stain?
magenta
What is wright’s stain and what color does it stain?
is a classic stain used for blood and it stains methylene blue and eosin
What is trichrome used for? How does it stain?
it is used to distinguish collagen in the extracellular matrix.
It stains blue
What is silver stain used for?
type 3 collagen or reticular fibers
What does Masson trichrome stain for?
type 1 collagen
What stain is being used here?
trichrome
What stain is being used here?
Silver stain
What type of microscopy is being used here?
does it use staining?
what happens with the light when it passes through an organelle?
phase contrast
no staining
cellular component diffract light
what type of microscopy is used here?
what happens when light hits the organelle?
bright field
light passes through the organelle
What does confocal microscopy use?
shines a laser light
What type of microscopy is this?
what type of medium does it use?
transmission electron microscopy
osmium tetroxide
how does scanning electrom microscope work?
specimen coated with silver and electron beam is shone on it, electrons bounce off.
what is immunohistochemistry?
antibodies produced against specific antigen, they stick to the antigen
what is an autoradiography? what can it be used for?
living tissue exposed to thymidine, then incorporated into replicating cells.
can be used to tag antibodies.
