1st powerpoint

Question 1

When you fixate a specimen, you have to first remove what?

Answer 1

degradative enzymes, at least neutralize them

Question 2

To fixate a specimen to look at it under a light microscope, you would utilize what solution?

Answer 2

formalin (formaldehyde)

Question 3

To fixate a specimen to look at it under a electron microscope, you would utilize what solution?

Answer 3

osmium tetroxide

or

glutaraldehyde

Question 4

What are the two most common stains?

Answer 4

Hematoxilin and Eosin

Question 5

What chrage does hematoxilin have?

Answer 5

its a basic dye

Question 6

What charge does Eosin have?

Answer 6

its an acidic dye; +

Question 7

What structures will hematoxilin stain?

Answer 7

it will stain acidic structures; RER, nucleoli, heterochromatin

Question 8

What structures will be stained with eosin?

Answer 8

basic structures; mitochondria,

Question 9

What color does hematoxilin stain?

Answer 9

blue

Question 10

what color does eosin stain?

Answer 10

red

Question 11

What are frozen sections used for?

Answer 11

to examine tissue rapidly

Question 12

When you stain, what does the staining do to observe a structure(s)?

Answer 12

it disrupts the light beam in order to view a structure

Question 13

In a cell, using eosin and hematixilin, what organelle will stain blue and what will stain red?

Answer 13

hematoxilin stains nuclei blue

eosin stains cytoplasm red

Question 14

What is PAS used for?

Answer 14

for staining glycoproteins

Question 15

What color does PAS stain?

Answer 15

magenta

Question 16

What is wright’s stain and what color does it stain?

Answer 16

is a classic stain used for blood and it stains methylene blue and eosin

Question 17

What is trichrome used for? How does it stain?

Answer 17

it is used to distinguish collagen in the extracellular matrix.

It stains blue

Question 18

What is silver stain used for?

Answer 18

type 3 collagen or reticular fibers

Question 19

What does Masson trichrome stain for?

Answer 19

type 1 collagen

Question 20

What stain is being used here?

Answer 20

trichrome

Question 21

What stain is being used here?

Answer 21

Silver stain

Question 22

What type of microscopy is being used here?

does it use staining?

what happens with the light when it passes through an organelle?

Answer 22

phase contrast

no staining

cellular component diffract light

Question 23

what type of microscopy is used here?

what happens when light hits the organelle?

Answer 23

bright field

light passes through the organelle

Question 24

What does confocal microscopy use?

Answer 24

shines a laser light

Question 25

What type of microscopy is this? what type of medium does it use?

Answer 25

transmission electron microscopy osmium tetroxide

Question 26

how does scanning electrom microscope work?

Answer 26

specimen coated with silver and electron beam is shone on it, electrons bounce off.

Question 27

what is immunohistochemistry?

Answer 27

antibodies produced against specific antigen, they stick to the antigen

Question 28

what is an autoradiography? what can it be used for?

Answer 28

living tissue exposed to thymidine, then incorporated into replicating cells. can be used to tag antibodies.