12th Page Flashcards
What is the approximate molecular weight of a light chain?
~25 kDa
How many amino acids are in a light chain?
~220 amino acids
What are the two types of light chains?
Kappa (k) and Lambda (λ) chains
Which chromosome is the Kappa light chain located on?
Chromosome 2
Which chromosome is the Lambda light chain located on?
Chromosome 22
Are both Kappa and Lambda light chains found in all 5 classes of immunoglobulin?
Yes, but only one type is present in a given molecule.
What is the approximate molecular weight of a heavy chain?
~50 - 70 kDa
How many amino acids are in a heavy chain?
~440 amino acids
Which chromosome is the heavy chain located on?
Chromosome 14
Ig type in Heavy Chain (GAMED)
They are unique to each class and give each Ig type its name: IgG, IgA, IgM, IgE, IgD.
What is the difference between allotype and idiotype?
Allotype refers to variations in constant regions (Gm groups), while idiotype refers to variations in variable regions.
Same heavy chain for each class/ H chain that is unique to each antibody
Isotype
Hinge region in Heavy chain is located where?
It is located between CH1 and CH2.
What is a characteristic finding in the urine of a patient with multiple myeloma?
BJP - Bence Jones Proteins
Light chains are found in the urine.
What are the two distinct regions contained in each light and heavy chain?
Variable (V) regions and Constant (C) regions.
How many amino acids of the N-terminal in a light chain are in variable region
First 110 amino acids
Variable region is also called?
Complimentary Determining region
Hypervariable regions
How many constant regions are in a light chain?
Single constant region.
How many constant regions are typically found in heavy chains?
3 - 4 constant regions.
What forms the basis of antibody classification?
Constant regions.
Domains
regions in Ab molecule
Components of IgG, IgA, IgD
L-1:1
H-1:3
The light chain has 2 domains: 1 VL and 1 CL.
The heavy chain has 4 domains: 1 VH and 3 CH.
What are the components of IgM and IgE?
L-1:1
H-1:4
1 light chain (2 domains: 1 VL and 1 CL)
1 heavy chain (5 domains: 1 VH and 4 CH).
Proteolytic/Chemical treatment of immunoglobulins.
Pepsin
Papain
Mercaptoethanol
What Ig has monomer structure?
Ig GADE
What is the structure of IgA?
Monomer and Dimer