Tests Flashcards
Patient serum and commercial sheep blood cells
coated with the appropriate antigen are required; (+) no reaction
INDIRECT HEMAGGLUTINATION (IHA)
Uses whole pathogens as a source of antigens
FLOCCULATION OR AGGLUTINATION
The patient serum is added to known cultured
parasites. This mixture is observed for the presence of
parasite clumping.
FLOCCULATION OR AGGLUTINATION
Bentonite or latex or the organism
FLOCCULATION OR AGGLUTINATION
USES: Leishmaniasis, Chaga’s disease
FLOCCULATION OR AGGLUTINATION
Detect antibodies against malarial parasites
INDIRECT FLUORESCENT ANTIBODY (IFA)
What is the antigen used in INDIRECT FLUORESCENT ANTIBODY (IFA)?
Plasmodium schizonts
A sandwich assay
INDIRECT FLUORESCENT ANTIBODY (IFA)
Ab and Ag are allowed to diffuse independently through a gel
Gel diffusion by ouchterlony
Ag (-) will go to anode, Ab (+) will go to cathode
COUNTERCURENT ELECTROPHORESIS
Requires concentrated antigens and takes several days
before results are available
GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP)
GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP) detects?
Detect amebiasis and fascioliasis infections
For detecting and quantifying soluble substances like proteins, peptides, and AB
ENZYME -LINKED IMMUNOSORBENT ASSAY (ELISA)
Modification of the standard ELISA for increased
sensitivity and specificity in detecting antibodies of
the IgM class to Toxoplasma
Double sandwich ELISA
Determine the concentration of an unknown ab in the sample
Double sandwich ELISA
In ELISA, The addition of a suitable substrate generates a
visible color reaction.
For taxoplasmosis
SABIN-FELDMAN DYE TEST
Toxoplasma trophozoites cant be stain by methylene blue
SABIN-FELDMAN DYE TEST
What can actively take up methylene blue?
Toxoplasma tachyzoites
Test for serum antibody for schistosoma
CIRCUMOVAL PRECIPITIN TEST (COPT)
What does circumoval precipitin test detect?
Schistosoma haematobium, Schistosoma japonicum
Competitive assay technique where the reagen and Ab is used in the limited amount as compared to the analyte Ag
Radioimmunoassay
Radioimmunoassay specific antibody is labelled with
Tracer isotopes
Determines the presence of true allergic reaction
RADIOALLERGOSORBENT TEST (RAST
test to detect specific IgE antibodies to suspected or known allergens for the purpose of guiding a diagnosis about allergy.
Radioallergosorbent test
What sample can you get for western blot?
CSF AND SREUM
Demonstrates antibodies to specific antigens of known molecular size
Western blot
Use for giardiasis
Western blot
For blood test
Complement fixation
What animal is used for Leishmania?
Hamster
Guinea pigs and white rats are used for.
Trypanosoma gambiense and Trypanosoma rhodesiense
White mice only is for?
Trypanosoma cruzi
Mice and rats are for?
Toxoplasma
Peritonial fluid is for
Toxoplasma
Method of feeding an animal with the parasites
Xenodiagnosis
Determine and quantify the transmission of a specific parasite to a host
Xenodiagnosis
What bugs are used for xenodiagnosis?
Laboratory-reared triatomid bugs
What parasite do you detect for xenodiagnosis
Trypanosoma cruzi
Specimens for Leishmania
Ulcers, lymph nodes, spleen, liver
Uses of complement fixation
Leishmaniasis, chaga’s disease, pneumocystosis, paragonimiasis
Used of indirect hemagglutination
Amoebiasis, chaga’s disease, malaria, toxoplasmosis, hydatid cyst, filariasis
Coated with Anti-human IgG antibody conjugated with enzyme is added
ELISA
for increased
sensitivity and specificity in detecting antibodies of
the IgM class to Toxoplasma
Antibody capture or double sandwhich elisa
Plastic well are coated with anti-human IgM
antibodies
Antibody Capture or Double Sandwich ELISA
Can take up methylene blue
Toxoplasma tachyzoites
Cannot take up methylene blue
Toxoplasma trophozoites
Make use of schistosoma eggs as antigen
Circumoval precipitin test
Fingerlike precipitates around the eggs (2 - 24 hours)
CIRCUMOVAL PRECIPITIN TEST (COPT)
