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PARASIT > Tests > Flashcards

Tests Flashcards

1
Q

Patient serum and commercial sheep blood cells
coated with the appropriate antigen are required; (+) no reaction

A

INDIRECT HEMAGGLUTINATION (IHA)

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2
Q

Uses whole pathogens as a source of antigens

A

FLOCCULATION OR AGGLUTINATION

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3
Q

The patient serum is added to known cultured
parasites. This mixture is observed for the presence of
parasite clumping.

A

FLOCCULATION OR AGGLUTINATION

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4
Q

Bentonite or latex or the organism

A

FLOCCULATION OR AGGLUTINATION

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5
Q

USES: Leishmaniasis, Chaga’s disease

A

FLOCCULATION OR AGGLUTINATION

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6
Q

Detect antibodies against malarial parasites

A

INDIRECT FLUORESCENT ANTIBODY (IFA)

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7
Q

What is the antigen used in INDIRECT FLUORESCENT ANTIBODY (IFA)?

A

Plasmodium schizonts

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8
Q

A sandwich assay

A

INDIRECT FLUORESCENT ANTIBODY (IFA)

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9
Q

Ab and Ag are allowed to diffuse independently through a gel

A

Gel diffusion by ouchterlony

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10
Q

Ag (-) will go to anode, Ab (+) will go to cathode

A

COUNTERCURENT ELECTROPHORESIS

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11
Q

Requires concentrated antigens and takes several days
before results are available

A

GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP)

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12
Q

GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP) detects?

A

Detect amebiasis and fascioliasis infections

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13
Q

For detecting and quantifying soluble substances like proteins, peptides, and AB

A

ENZYME -LINKED IMMUNOSORBENT ASSAY (ELISA)

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14
Q

Modification of the standard ELISA for increased
sensitivity and specificity in detecting antibodies of
the IgM class to Toxoplasma

A

Double sandwich ELISA

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15
Q

Determine the concentration of an unknown ab in the sample

A

Double sandwich ELISA

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16
Q

In ELISA, The addition of a suitable substrate generates a

A

visible color reaction.

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17
Q

For taxoplasmosis

A

SABIN-FELDMAN DYE TEST

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18
Q

Toxoplasma trophozoites cant be stain by methylene blue

A

SABIN-FELDMAN DYE TEST

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19
Q

What can actively take up methylene blue?

A

Toxoplasma tachyzoites

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20
Q

Test for serum antibody for schistosoma

A

CIRCUMOVAL PRECIPITIN TEST (COPT)

21
Q

What does circumoval precipitin test detect?

A

Schistosoma haematobium, Schistosoma japonicum

22
Q

Competitive assay technique where the reagen and Ab is used in the limited amount as compared to the analyte Ag

A

Radioimmunoassay

23
Q

Radioimmunoassay specific antibody is labelled with

A

Tracer isotopes

24
Q

Determines the presence of true allergic reaction

A

RADIOALLERGOSORBENT TEST (RAST

25
Q

test to detect specific IgE antibodies to suspected or known allergens for the purpose of guiding a diagnosis about allergy.

A

Radioallergosorbent test

26
Q

What sample can you get for western blot?

A

CSF AND SREUM

27
Q

Demonstrates antibodies to specific antigens of known molecular size

A

Western blot

28
Q

Use for giardiasis

A

Western blot

29
Q

For blood test

A

Complement fixation

30
Q

What animal is used for Leishmania?

A

Hamster

31
Q

Guinea pigs and white rats are used for.

A

Trypanosoma gambiense and Trypanosoma rhodesiense

32
Q

White mice only is for?

A

Trypanosoma cruzi

33
Q

Mice and rats are for?

A

Toxoplasma

34
Q

Peritonial fluid is for

A

Toxoplasma

35
Q

Method of feeding an animal with the parasites

A

Xenodiagnosis

36
Q

Determine and quantify the transmission of a specific parasite to a host

A

Xenodiagnosis

37
Q

What bugs are used for xenodiagnosis?

A

Laboratory-reared triatomid bugs

38
Q

What parasite do you detect for xenodiagnosis

A

Trypanosoma cruzi

39
Q

Specimens for Leishmania

A

Ulcers, lymph nodes, spleen, liver

40
Q

Uses of complement fixation

A

Leishmaniasis, chaga’s disease, pneumocystosis, paragonimiasis

41
Q

Used of indirect hemagglutination

A

Amoebiasis, chaga’s disease, malaria, toxoplasmosis, hydatid cyst, filariasis

42
Q

Coated with Anti-human IgG antibody conjugated with enzyme is added

A

ELISA

43
Q

for increased
sensitivity and specificity in detecting antibodies of
the IgM class to Toxoplasma

A

Antibody capture or double sandwhich elisa

44
Q

Plastic well are coated with anti-human IgM
antibodies

A

Antibody Capture or Double Sandwich ELISA

45
Q

Can take up methylene blue

A

Toxoplasma tachyzoites

46
Q

Cannot take up methylene blue

A

Toxoplasma trophozoites

47
Q

Make use of schistosoma eggs as antigen

A

Circumoval precipitin test

48
Q

Fingerlike precipitates around the eggs (2 - 24 hours)

A

CIRCUMOVAL PRECIPITIN TEST (COPT)

PARASIT (29 decks)

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