Tests

Question 1

Patient serum and commercial sheep blood cells
coated with the appropriate antigen are required; (+) no reaction

Answer 1

INDIRECT HEMAGGLUTINATION (IHA)

Question 2

Uses whole pathogens as a source of antigens

Answer 2

FLOCCULATION OR AGGLUTINATION

Question 3

The patient serum is added to known cultured
parasites. This mixture is observed for the presence of
parasite clumping.

Answer 3

FLOCCULATION OR AGGLUTINATION

Question 4

Bentonite or latex or the organism

Answer 4

FLOCCULATION OR AGGLUTINATION

Question 5

USES: Leishmaniasis, Chaga’s disease

Answer 5

FLOCCULATION OR AGGLUTINATION

Question 6

Detect antibodies against malarial parasites

Answer 6

INDIRECT FLUORESCENT ANTIBODY (IFA)

Question 7

What is the antigen used in INDIRECT FLUORESCENT ANTIBODY (IFA)?

Answer 7

Plasmodium schizonts

Question 8

A sandwich assay

Answer 8

INDIRECT FLUORESCENT ANTIBODY (IFA)

Question 9

Ab and Ag are allowed to diffuse independently through a gel

Answer 9

Gel diffusion by ouchterlony

Question 10

Ag (-) will go to anode, Ab (+) will go to cathode

Answer 10

COUNTERCURENT ELECTROPHORESIS

Question 11

Requires concentrated antigens and takes several days
before results are available

Answer 11

GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP)

Question 12

GEL DIFFUSION (OUCHTERLONY) AND COUNTERCURENT ELECTROPHORESIS (CEP) detects?

Answer 12

Detect amebiasis and fascioliasis infections

Question 13

For detecting and quantifying soluble substances like proteins, peptides, and AB

Answer 13

ENZYME -LINKED IMMUNOSORBENT ASSAY (ELISA)

Question 14

Modification of the standard ELISA for increased
sensitivity and specificity in detecting antibodies of
the IgM class to Toxoplasma

Answer 14

Double sandwich ELISA

Question 15

Determine the concentration of an unknown ab in the sample

Answer 15

Double sandwich ELISA

Question 16

In ELISA, The addition of a suitable substrate generates a

Answer 16

visible color reaction.

Question 17

For taxoplasmosis

Answer 17

SABIN-FELDMAN DYE TEST

Question 18

Toxoplasma trophozoites cant be stain by methylene blue

Answer 18

SABIN-FELDMAN DYE TEST

Question 19

What can actively take up methylene blue?

Answer 19

Toxoplasma tachyzoites

Question 20

Test for serum antibody for schistosoma

Answer 20

CIRCUMOVAL PRECIPITIN TEST (COPT)

Question 21

What does circumoval precipitin test detect?

Answer 21

Schistosoma haematobium, Schistosoma japonicum

Question 22

Competitive assay technique where the reagen and Ab is used in the limited amount as compared to the analyte Ag

Answer 22

Radioimmunoassay

Question 23

Radioimmunoassay specific antibody is labelled with

Answer 23

Tracer isotopes

Question 24

Determines the presence of true allergic reaction

Answer 24

RADIOALLERGOSORBENT TEST (RAST

Question 25

test to detect specific IgE antibodies to suspected or known allergens for the purpose of guiding a diagnosis about allergy.

Answer 25

Radioallergosorbent test

Question 26

What sample can you get for western blot?

Answer 26

CSF AND SREUM

Question 27

Demonstrates antibodies to specific antigens of known molecular size

Answer 27

Western blot

Question 28

Use for giardiasis

Answer 28

Western blot

Question 29

For blood test

Answer 29

Complement fixation

Question 30

What animal is used for Leishmania?

Answer 30

Hamster

Question 31

Guinea pigs and white rats are used for.

Answer 31

Trypanosoma gambiense and Trypanosoma rhodesiense

Question 32

White mice only is for?

Answer 32

Trypanosoma cruzi

Question 33

Mice and rats are for?

Answer 33

Toxoplasma

Question 34

Peritonial fluid is for

Answer 34

Toxoplasma

Question 35

Method of feeding an animal with the parasites

Answer 35

Xenodiagnosis

Question 36

Determine and quantify the transmission of a specific parasite to a host

Answer 36

Xenodiagnosis

Question 37

What bugs are used for xenodiagnosis?

Answer 37

Laboratory-reared triatomid bugs

Question 38

What parasite do you detect for xenodiagnosis

Answer 38

Trypanosoma cruzi

Question 39

Specimens for Leishmania

Answer 39

Ulcers, lymph nodes, spleen, liver

Question 40

Uses of complement fixation

Answer 40

Leishmaniasis, chaga’s disease, pneumocystosis, paragonimiasis

Question 41

Used of indirect hemagglutination

Answer 41

Amoebiasis, chaga’s disease, malaria, toxoplasmosis, hydatid cyst, filariasis

Question 42

Coated with Anti-human IgG antibody conjugated with enzyme is added

Answer 42

ELISA

Question 43

for increased sensitivity and specificity in detecting antibodies of the IgM class to Toxoplasma

Answer 43

Antibody capture or double sandwhich elisa

Question 44

Plastic well are coated with anti-human IgM antibodies

Answer 44

Antibody Capture or Double Sandwich ELISA

Question 45

Can take up methylene blue

Answer 45

Toxoplasma tachyzoites

Question 46

Cannot take up methylene blue

Answer 46

Toxoplasma trophozoites

Question 47

Make use of schistosoma eggs as antigen

Answer 47

Circumoval precipitin test

Question 48

Fingerlike precipitates around the eggs (2 - 24 hours)

Answer 48

CIRCUMOVAL PRECIPITIN TEST (COPT)