Fa Flashcards

1
Q

simplest and most frequently used

A

DFS

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2
Q

for the observation of the motility of trophozoites

A

0.85% NaCl

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3
Q

doesn’t demonstrate glycogen vacuole and nuclei

A

0.85% NaCl

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4
Q

cysts appear glistening and refractile

A

0.85% NaCl

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5
Q

for protozoan cysts and helminth ova
kill the trophozoites and helminth larva

A

D’antonis solution/Lugol’s Iodine

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6
Q

2 structures seen in cyst in D’antonis

A

Chromatodal bars and glycogen vacuole

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7
Q

Uses of coverslip

A
  1. to avoid drying of the preparation
  2. to avoid fogging and contamination of objectives
  3. to avoid rapid loss of the stain
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8
Q

Appropriate for formed or semiformed stool

A

Kts

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9
Q

For Emulsification of stool

A

Distilled water

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10
Q

Clearing agent in kts

A

Glycerin

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11
Q

Stain inkts

A

3% malachite green

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12
Q

Coverslip in kts

A

Cellophane

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13
Q

Clearing time of kts in room temp

A

1 hour

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14
Q

Clearing time of kts in dry incubation

A

20-30mins

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15
Q

Satisfactory for all kinds of helminth eggs

A

Kts

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16
Q

For large scale examination

A

Kts

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17
Q

Unsuitable for protozoan cysts and trophozoites

A

Kts

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18
Q

Detection of small number of parasites not detected using DFS

A

Concentration techniques

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19
Q

Protozoan cysts, helminth eggs and larvae

A

Sedimentation method

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20
Q

Less efficient than flotation: because protozoan cysts and
many eggs may not be seek.

A

Sedimentation method

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21
Q

Concentrate eggs, larvae and cysts of parasites, operculated and schistosomal eggs

A

Formalin-ether technique

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22
Q

Preservative and fixative

A

Formalin

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23
Q

How many minutes in formalin before centrifugation

A
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24
Q

Dissolves lipids and other colloidal material

25
Material can be examined hours or even days after preparation
FECT
26
Not applicable for protozoan cysts
Acid ether sedimentation
27
we may observe helminth eggs and larvae
Acid ether sedimentation
28
Cleraing agent in acid ether sedimentation
29
Not recommended because it is time consuming
Simple sedimentation
30
For blood only to detect microfilariae
Knott concentration technique
31
Helminths eggs and protozoan cysts may either float or sink depending on their specific gravitate
Flotation technique
32
Superior than sedimentation
Flotation method
33
Helminth eggs, protozoan cysts and larvae specific gravity
1.05-1.15
34
Chemical solutions specific gravity
1.12 to 1.21
35
Optimal time for examination of flotation method
5-20 minutes
36
Zinc sulfate centrifugal flotation technique specific gravity
1.18
37
80% effective in detecting eggs and cysts in light infections
ZINC SULFATE CENTRIFUGAL FLOTATION TECHNIQUE
38
Hookworm SG
1.005
39
Ascaris (fertile) sg
1.110
40
Trichuris sg
1.150
41
Giardia sg
1.060
42
E. Histolytica sg
1.070
43
Laboratory test for occult blood
Benzidine Guiaic Hematest
44
Meat free diet for how many days prior to occult blood test
3-5 days
45
False pos or false neg myoglobin in meat
False pos
46
False pos or false neg vitamin c
False neg
47
blood which is not visible to the naked eye and can only be detected by chemical means only
Occult blood
48
due to hemorrhagic disorder, ulcers and contamination
Rbc
49
PRESENT IN BACTERIAL OR PARASITIC INFECTION
Macrophages
50
indicative of inflammation
Wbc
51
Fungi in occult blood
Candida spp
52
Pseudoparasite in occult blood
Plant cells, pollen grains and spores
53
Increased during inflammation of the lower bowel
Epithelial cells
54
Contaminated by urine in occult blood
Calcium oxalate and triple phosphate crystls
55
Similar to helminth ova in occult blood
Plant fibers Root hair Animal cells
56
Mistaken by trichuris trichuria or karvae
Charcot latyden crystals
57
Color of glycogen vacuole
Mahigany brown
58
Uses specific gravity and centrifugation
Sedimentation method