Fa Flashcards
simplest and most frequently used
DFS
for the observation of the motility of trophozoites
0.85% NaCl
doesn’t demonstrate glycogen vacuole and nuclei
0.85% NaCl
cysts appear glistening and refractile
0.85% NaCl
for protozoan cysts and helminth ova
kill the trophozoites and helminth larva
D’antonis solution/Lugol’s Iodine
2 structures seen in cyst in D’antonis
Chromatodal bars and glycogen vacuole
Uses of coverslip
- to avoid drying of the preparation
- to avoid fogging and contamination of objectives
- to avoid rapid loss of the stain
Appropriate for formed or semiformed stool
Kts
For Emulsification of stool
Distilled water
Clearing agent in kts
Glycerin
Stain inkts
3% malachite green
Coverslip in kts
Cellophane
Clearing time of kts in room temp
1 hour
Clearing time of kts in dry incubation
20-30mins
Satisfactory for all kinds of helminth eggs
Kts
For large scale examination
Kts
Unsuitable for protozoan cysts and trophozoites
Kts
Detection of small number of parasites not detected using DFS
Concentration techniques
Protozoan cysts, helminth eggs and larvae
Sedimentation method
Less efficient than flotation: because protozoan cysts and
many eggs may not be seek.
Sedimentation method
Concentrate eggs, larvae and cysts of parasites, operculated and schistosomal eggs
Formalin-ether technique
Preservative and fixative
Formalin
How many minutes in formalin before centrifugation