Quiz 2 Flashcards
The primary specimen used
Stool
For intestinal protozoans, nematodes and helminthes
Stool
Rotten egg smell
Giardia
Urine is used for the recovery of?
Trichomonas vaginalis and Schistosoma haematobium
Schistosoma haematobium’s habitat is?
Kidney
Sputum is for identification of what parasite?
Paragonimus westermani
Paragonimus westermani is lodged in the.
Lungs
This must be dugested in 4-5% sodium hydroxide
Sputum
For malarial parasites, filarial worms, Leishmania and Trypanosoma
Blood
Cerebrospinal fluid is used for identification of what species?
Acanthamoeba species
Collection of CSF?
Lumbar tap
Liver aspirate is used for?
Hyadid cyst and liver amoebic abscess
Used for Giardiasis and strongyloidiasis infection
Duodenum aspirate
duodenal contents
collected for Giardia and Strongylodes
Duodenal drainage or “string test”
Sigmoidoscopy is used in?
Schistosomiasis, amoebiasis, balantidiasis, shigellosis
Broncho-alveolar lavage
Paragonimus westermani
Rectal
Granuloma secondary to schistosomiasis
Vaginal swab is used for?
Trichomonas vaginalis
Perianal swab is used for?
Enterobius vermicularis and Taenia
Scotch tape method?
Enterobius vermicularis
Lays its eggs in the anus at night time
Enterobius vermicularis
Found in the muscle
Trichinella spiralis
for diagnosis of Trypanosomes and microfilaria
Fresh water smears
for the study of the morphology of the parasites and the blood cells
Thin dry smears
used for malaria survey among patients with chronic infections or who are undergoing anti-malaria therapy.
Thick dry smear
most preferred stain
Giemsa
Buffer pH of giemsa
pH 7-7.2
Staining time of giemsa
30 mins
How much buffered water in giemsa?
49 mL
If stain is too dark?
Acidic pH
If stain is too red?
Alkaline pH
it is used to stain blood smears in the detection of blood parasites
Wright’s stain
is a mixture of methylene blue, and eosin dye, prepared in alcohol medium and diluted with buffer or distilled water during staining procedure to neutralize
Leishmann stain
it is a histological method for staining of blood smears
Field’s stain
is used as a fluorescent staining agent to detect the presence of malaria parasite in blood cultures and other bodily fluids
Acridine orange
standard method: laboratories under the National Malaria Eradication Programme in India
Jaswant Singh Battacharya stain for thick and thin films
used for most of the original morphological descriptions of intestinal protozoa found in humans.
Iron hematoxylin stain
diagnosis of Trichomonas
Merthiolate-iodine-formaldehyde fixative stain
an acid dye, used as a fat and general tissue stain, and to stain protozoa in fecal smears or in tissues.
Chlorazol black E
modification of the chlorazol black E staining technique
Modified Kohn’s
all-purpose (amoeba, flagellates)
Wheatley Trichrome
Amoeba cyst stain
Methenamine silver
Microsporidium stain
Fluroorescent staining
a differential stain that is used to variably stain the
various components of the cells and it can be used to study the adherence of
pathogenic bacteria to the human cells
Leishmann Stain
The stain distinguishes easily between blood cells and became
widely used for performing differential white blood cell counts, which
are routinely ordered when infections are expected.
Wright’s stain
Thin films of blood are
fixed with methanol to preserve the red cell morphology so that the
relationship between parasites to the red cells can be seen clearly.
Wright’s stain
Fixative of wright’s stain
Methanol
It is used for
staining thick blood films in order to discover malarial parasites
Field’s stain
Field’s stain A
methylene blue and Azure 1 dissolved in
phosphate buffer solution;
Field’s stain B
Eosin Y in buffer solution
NMEPII
National Malaria Eradication Programme in
India
Parasite that cannot survive outside of a host
Obligatory Parasite
Parasite that is capable of existing independently of a host
Facultative parasite
Parasite that is established inside of a host
Endoparasite
Parasite that is established in or
on the exterior surface of a host
Ectoparasite
Host other than the normal one that is harboring a parasite
Accidental host
Host in which the adult sexual phase of parasite development occurs
Definitive host
Host in which the larval asexual phase of parasite development occurs
Intermediate host
Host harboring parasites that are parasitic for humans and from which humans may become infected
Reservoir
Host responsible for transferring a parasite from one location to another
Transport host
Parasite-harboring host that is not exhibiting any clinical symptoms but can infect others
Carrier
Tissue aspirate mode of collection
Endoscopy