protozoa Flashcards
fusion of two cells or their nuclei in
reproduction
Sexual/ Syngamy:
for osmotic pressure and elimination of
waste product
Contraction vacuoles:
➢ Complete unit
➢ With specialized cells
unicellular
➢ Amitotic: single division
➢ Mitotic: repeated binary fission of nucleus
producing two daughter cells
Asexual reproduction
repeated binary fission of nucleus
producing two daughter cells
mitotic
single division
amitotic
locomotion of protozoa
organelles
inner , finely granular protoplasm primarily for
storage synthesis and digestion of food
Cytoplasm/ endoplasm
For locomotion, respiration and discharge or
metabolic wastes
ectoplasm
semi-permeable
membrane
● For intake and outtake of food
as well as for secretion or
secretory
Plasma Membrane
for regulation of osmotic pressure
contractile vacuoles
cell mouth, located laterally
at the anterior end
Cytostome-
With thin ectoplasm which is primarily for ingestion,
excretion, protection and movement
cytoplasm
cell anus
Cytophage
where flagellum arises
kinetoplast (mastigophora)
Maintenance and reproduction of life
nucleus
for nutrition and reproduction
granular voluminous endoplasm
Classification of protozoa
movement
direct; oxygen os from
enzymatic reactions
aerobic
for parasitic forms of protozoa
anerobic
opening for extrusion
nutrition; ingestion = cytopyge
types of nutrition
Absorption
ingestion
types of excretion
osmotic pressure
diffusion
precipitation
usually the infective stage
Resistant membranous wall for cervical
means of survival
reproduction
sexual union of 2 cells
syngammy
Fission through mitotic and amitotic
● May be grown in vitro
➢ Blood, serum, but it require
asexual
feeding stage/ reproducing stage/ moving stage
Trophozoite
non-feeding stage/ non-reproducing stage/ nonmoving stage
cyst
Usually resistant to adverse conditions
cyst
infective form of protozoa
CYST
invertebrae host
arthropods
- host to host without any cyclic development
direct transmisson
what development happens inside the vector and what vector did it happen into>
Cyclic development; mosquito vector
There is a vehicle (foor or water) or a vector. So when
the protozoa needs a vector, this is with a cyclic development.
indirect transmission
3 types of pathology of protozoa
Invasion
immune response
toxic products
destruction of cells
imvasion
laboratory diagnosis in cases of malaira
blood smear
diagnosis:
isolation of amoeba and flagellates
Concentration studies
amoeba superclass
Sarcodina
amoeba class
rhizopodea
THE AMOEBA UNDERGO ENCYSTATION EXCEPT FOR
ENTAMOEBA GINGIVALIS
the conversion of trophozoites into cyst form.
encystation
encystation usually occurs in?
small intestine
. The ingestion of the____ completes the
typical intestinal amebic life cycle.
infective cysts
leads to the trophozoite formation
excystation
leads to the trophozoite formation.
excystation
excystation occurs in
ileocecal of the intestines
Most pathogenic intestinal amoeba in man
Entamoeba histolytica
habitat of E.histo
large intestine, liver, lungs and brain
amoebas undergoes encystation except for?
entamoeba gingibavalis
E.histo is also known as
Amoeba Dysenteriae, Entamoeba
Dysenteriae, Entamoeba Dysentery and Entamoeba
Tetragena”
infective stage of E.histp
cyst
Chromatoidal bodies: has rounded ends giving it a
sausage shape appearance
E.histo cyts
E.histo;
❖ Ectoplasm is thick
❖ Red blood cells present
❖ No bacteria or foreign material
❖ Karyosome is centrally located
❖ Pseudopodia is fingerlike
❖ Progressive movement for the trophozoite stage of E.
histolytica
Trophozoite
E.histo:
Progressive movement for the trophozoite stage of E.
histolytica
Trophozoite
E. histo: Colorless, round or oval
❖ Smaller than trophozoite but bigger than the cyst
❖ Devoid of food inclusion
❖ Movement is sluggish
Precystic
optimum temp and pH of E.histo cyst
37 deg cel; pH 7
chromatoidal bodies has rounded eggs giving it a sausage shape appearance
E.histlytica
thermal death point of E.histo cyst
50 deg cel
resistant to urine
E. histo cyts
how many nuclei in mature and immature cyst if E.histo
4 in mature
1-2 in immature
movement of E.histo
Active
Progressice
DIrectional
Fingerlike
Disease E.histo;
There are symptoms that are apparent
Luminal amoebiasis
● the trophozoites would invade
the intestinal mucosa to produce dysentery or
ameboma that can spread in
the blood to give extra
intestinal lesions like liver
abscess.
Invasive amoebiasis
asymptomatic infection
Luminal amoebiasis specifically the E.dispar
this is useful in
differentiating Entamoeba histolytica from Entamoeba
dispar because these two are morphologically
indistinguishable.
Polymerase chain Reaction
Not useful for demonstrating trophozoites
Fecalysis; E.histo
Fresh stool: wet mounts (with or without the iodine stain)
and permanently stained preparations
Fecalysis; e histo
. It can also help in the differentiation of
luminal infection caused by Entamoeba dispar from
invasive amoebiasis caused by Entamoeba histolytica.
Polymerase Chain Reaction
most useful in patient with
extraintestinal disease (i.e. amebic liver abscess) when
organisms are not generally found on stool examination
antibody detection
: useful as an adjunct to microscopic
diagnosis in detecting parasites and to distinguish between
pathogenic and non-pathogenic infections (between E.
histolytica and E. dispar infections
Antigen detection