Quiz 3 Flashcards
Introduce erronous organism that will hinder examination like pseudoparasit
Soil
Destroys trophozoids
Urine and water
This produce discoloration of the stool
Barium and bismuth
This disrupts vision of viewing
Oil
Gallbladder dye clearing days
3 weeks
Barium and bismuth clearing time
7-10 days
Traditionally used to treat diarrhea to relieve soreness specially in patients undergoing radiologic therapy
Kaolin
Antibiotics clearing time
2-3 weeks
Stool type that us characterized by irritable bowel syndrome, constipation, diarrhea, abdominal cramps and obstruction of rectum
Ribbonlike stool
Stool that does not retain the bowel cast and due to trapped gases
Mushy stool
Stool due to excessive carbohydrate fermentation
Mushy stool
Trophozoid is found in what kind of stool?
Watery stool
Cyts is found in what kind of stool
Formed stool
Preparation of stained smears
prepared in the lab or purchased commercially.
Polyvinyl alcohol
Modified schaudinn’s fixative
Polyvinyl alcohol
The preserved sample can remain stable for several months
PVA
QC: cloudy soln
PVA
Inadequate to determine the morphology of helming eggs, larva, toxidia microsporidia
PVA
Difficult and expensive to dispose
PVA
Cannot be used in immunoassays, acid fast, safranin and chromotroph stain
PVA
Difficult and expensive to dispose
PVA
Prepared thru wet mounts
5-10% formalin
Prepared thru with or without iodine (direct fecal smear same with wet mounts)
5-10 % FORMALIN
Preserved cysts, helminth egg and larva but can distort the morphology of trophozoids
5-10 % FORMALIN
QC: temperature favors development of helminth eggs, 60 degrees celsius
5-10 % FORMALIN
Compatible with acid stain, safranin and chromotroph stain
5-10 % FORMALIN
Can be used with immunoassay kits
5-10 % FORMALIN
Used in field testing or surveys
MERTHIOLATE-IODINE-FORMALIN (MIF
Used for concentration procedures
MERTHIOLATE-IODINE-FORMALIN (MIF
Dye of MIF
Lugol’s iodine
Solution of MIF
9.4mL of MF + 0.6 mL of lugol = 10mL + 1 gram of stool
Good for all stages and used for preserving cyst mtroph(?)
MERTHIOLATE-IODINE-FORMALIN (MIF
For fresh materials obtained from intestinal mucosal linings
SCHAUDINN’S
For permanent staining
SCHAUDINN’S
Good preservative for morphology of protozoan, trophozoids, and cysts
SCHAUDINN’S
Poor adhesion
SCHAUDINN’S
Does not contain mercuric chloride
SODIUM ACETATE – ACETIC ACID FORMALIN (
Liquid fixative with long shelf life
SODIUM ACETATE – ACETIC ACID FORMALIN (
Images of organisms are not sharp after staining
SODIUM ACETATE – ACETIC ACID FORMALIN (SAF)
Suitable for both concentration procedures and permanent stain solution
SODIUM ACETATE – ACETIC ACID FORMALIN (SAF)
(-): requires additive primarily albumin or glycerine
SODIUM ACETATE – ACETIC ACID FORMALIN (SAF)