Hemoflagellates Flashcards
Four stages of development
Leishmania
Leptomonad
Crithidia
Trypanosome
A.k.a. Amastigote stage
LEISHMANIA
Promastigote stage
LEPTOMONAD
Inner portion of the flagellum that is actually extend from the blepharoplast to the surface of the body
Axoneme
Epimastigote stage
CRITHIDIA
● Trypanomastigote stage
TRYPANOSOME
Stage: Reduce d when it
comes to size and does not have any flagellu m
Amastigote
Common name of amastigote
Leishmania form
Stage: The flagellum is anterior place and away from the cell body
Promastigote
Promastigote common name
Leptomonad form
stage: nucleus
is almost
centrally
located;
located
in the
anterior
part of
the
parasite
Epimastigote
common name of epimastigote
Crithidial form
Stage: Connected with
short angionating
membrane
Epimastigote
TRYPANOSOMA BRUCEI GAMBIENSE disease
West african sleeping sickness (97%)
mild african sleeping sickness
gambian trypanosomiasis
Stage: Flagella in the center of organism
Epimastigote
stage: Connected with
long angionating
membrane
Trypomastigote
stage: Flagella originates at posterior end
of the organism
Trypomastigote
Trypomastigote common name
Trypanoso
mal form
TRYPANOSOMA BRUCEI RHODESIENSE DISEASE
East african sleeping sickness
TRYPANOSOMA BRUCEI GAMBIENSE GEOGRAPHIC DISTRIBUTION
Central and West Africa
TRYPANOSOMA BRUCEI RHODESIENSE EPIDEMIOLOGICALLY RELEVANT ANIMAL/RESERVOIr
Domestic Cattles
TRYPANOSOMA BRUCEI GAMBIENSE main reservoir
● Human
● They can also be found in animals including the primates
TRYPANOSOMA BRUCEI RHODESIENSE
VECTOR
Glossina Species (Tsetse fly)
Glossina palpalis
GLossina morsitans
T.cruzi disease
Chaga’s disease
American Trypanosomiasis
TRYPANOSOMA BRUCEI GAMBIENSE VECTOR
Glossina Species (Tsetse fly)
Glossina palpalis
Glossina tachinoides
T.cruzi vector
Kissing bug or Assassin bug
Tritoma or Reduviidae
TRYPANOSOMA BRUCEI RHODESIENSE GEOGRAPHIC DISTRIBUTION
East and South Africa
alternatively known as sleepiness or
drowsiness; state of a strong desire to sleep or sleeping for
unusually long periods of time.
Somnolence-
chaga diseas discoverer
Carlos Ribeiro Justiniano Chagas
Disease Named from the Brazilian physician and researcher
who discovered chaga’s disease on 1609
Chaga’s disease
● Cyclical fever with a seeming remission
● Fever-free interval of at least two weeks
EPISODIC FEVER- RECURRENT FEVER
● Disease of the lymph nodes in which there are abnormal size
or consistency
● Lymphadenopathy of inflammatory types specifically, the
most common type is lymphadenitis producing swelling or
enlarged lymph nodes.
LYMPHADENOPATHY/ ADENOPATHY
enlargement of the posterior cervical
lymph nodes.
Winterbottom ‘s sign-
GAMBIAN TRYPANOSOMIASIS ● Systemic phase:
episodic (lasting from 1-6 days followed by
an asymptomatic period lasting several weeks)
earliest sign of Gambian Trypanosomiasis
● Characterized by hard painful lesion at the site of insect bite
CHANCRE
GAMBIAN TRYPANOSOMIASIS
● Incubation period:
14 days
vector of T.cruzi
Triatomid Bug
Kissing bug
Assassin bug
T.cruzi main habitat
Reticuloendothelial cells
Central nervous System
Gambian trypanosomiasis CNS symptomes:
Takes years to develop
Rhodesian trypanosomiasis CNS manifestations
develop 3-6 months
Usually appear 48 hours after the tsetse bite
TRYPANOSOMAL CHANCRE
changes around the eyes usually
characterized by swelling
ROMANAS SIGN
● Periorbital changes-
usually start at the end of the first year or beginning of the
second year
CHRONIC SLEEPING SICKNESS
itchy, painful inflammatory reaction at the site of the insect bite
● The chancre is actually uninjured (hardened) red or purple
nodule
● The nodule would usually measure 2-5 cm in diameter which
is accompanied by enlarge lymphs
TRYPANOSOMAL CHANCRE
Begins with local lesion (chagoma)
● Marked local edema within the region of the eye or within the
conjunctival sac
ROMANAS SIGN
usually seen in the early phase of African
trypanosomiasis specifically Trypanosoma brucei
rhodesiense and Trypanosoma brucei gambiense
Winterbottom’s sign
○ CNS invasion
○ Increased metal dullness & opathy
○ Tremors, hyperesthesia (abnormal increase
sensitivity to stimuli of any of the senses),
somnolence
CHRONIC SLEEPING SICKNESS
- commonly known as African
sleeping sickness
Trypanosoma brucei gambiense
inflammation of the mesenteric lymph
nodes or glands found in the abdomen
Adenitis- PATHOLOGY: CHAGA’S DISEASE
slightly painful reddish nodule at the site of
inoculation of the reduviid bug. This bug transmits the chaga’s
disease.(initial lesion)
Chagoma PATHOLOGY: CHAGA’S DISEASE
he esophagus and the
colon will already be involved in chronic cases
Esophagial or colonic involvement PATHOLOGY: CHAGA’S DISEASE
disease of the heart muscle that makes it
harder for the heart to pump blood to the rest of the body. It
can lead to heart failure.
Cardiomyopathy- PATHOLOGY: CHAGA’S DISEASE
Pre-orbital soft tissue and lymphoid swelling that
occurs when T. cruzi causes chaga’s disease
enters the conjunctiva of the eye.
Romanas sign
vector of T.cruzi
Reduviid bug
host of T.cruzi
Dasypus novencinctus
Opossums (Dildelphis species
unilateral conjunctivitis and orbital edema (if
portal of entry is the upper face or eye)
Romanas sign PATHOLOGY: CHAGA’S DISEAS
This is breeding of a clean bugs in
laboratories then after 2-3 weeks, their guts will be dissected
to determine the metacyclic trypanosomes.
Xenodiagnosis
- There is an abnormal increase in the
amount of lymphocytes in the cerebrospinal fluid. Thus,
lumbar tap is used for CSF then observed under the
microscope if there is an increase amount of lymphocytes in
CSF. It also measures increased protein content.
Lymphatic pleocytosis
L. braz disease
Espundia or MCL, Chiclero’s ulcer or New World
cutaneous leishmaniasis
L.trop vector
Phlebotomus spp. (sandfly)
P.papatasii
P.sargenti
LEISHMANIA TROPICA disease
Oriental sore or Old World Cutaneous
Leishmaniasis
L.bra: In the mid cut of the sandfly the amastigote would develop to
___ which would replicate profusely. These are
internally transmitted to the skin of the person bitten by the
sandfly
promastigote
L.bra vector
Phlebotomus spp. (sandfly)
nvolves the liver, spleen, and the bone marrow
VISCERAL LEISHMANIASIS