Tech Flashcards

1
Q

Cryptosporidium

A

SUGAR FLOTATION TECHNIQUE

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2
Q

We can isolate here the rounded oocysts and crescent
sporozoites

A

SUGAR FLOTATION TECHNIQUE/

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3
Q

uses sat. NaCl solution- 40g NaCl + 100 mL dist. H2O

A

WILLIE’S BRINE

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4
Q

4 consecutive negative results

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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5
Q

Collection: morning before the patient washes or defecates

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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6
Q

Enterobius vermicularis and Taenia

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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7
Q

Estimates worm burden

A

EGG COUNT TECHNIQUE

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8
Q

✓ Determines the degree of infection (light, moderate or heavy)

A

Egg Count Technique –

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9
Q

For recovery of hookworms, Ascaris and Trichuris

A

Egg Count Technique –

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10
Q

1.5 mg feces (667) = epg
2 mg feces (500) = epg

A

DIRECT SMEAR EGG COUNT

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11
Q

-0.1 N NaOH

A

STOLL’S EGG COUNTING TECHNIQUE DILUTION EGG
COUNT

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12
Q

-KTS FOR SCHISTOSOMES

A

THICK SMEAR EGG COUNT

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13
Q

FOR
SCHISTOSOMES

A

DILUTION-FILTRATION EGG COUNT

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14
Q

The counts can be reported as eggs per direct smears

A

DIRECT SMEAR EGG COUNT

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15
Q

Dilution egg count is by?

A

By Stoll and Hausheer

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16
Q

The Kato cellophane covered thick smear is modified by

A

Martin and Beaver

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17
Q

For simultaneous qualitative and quantitative diagnosis of the common intestinal nematode infections

A

THICK SMEAR EGG COUNT

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18
Q

For routine quantitative diagnosis of Schistosoma mansoni and Schistosoma japanicum infections.

A

THICK SMEAR EGG COUNT

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19
Q

DILUTION-FILTRATION EGG COUNT is by?

A

Bell

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20
Q

can be concentrated filtering a suspension
of feces through filter paper on which the eggs are stained and counted.

A

Schistosome eggs- DILUTION-FILTRATION EGG COUNT

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21
Q

is a laboratory tool used to determine a given parasite’s resistance to extant drug therapy.

A

Egg hatching assay

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22
Q

Fresh eggs are incubated from the parasite of interest and serial
dilutions of the drug of interest are applied

A

Egg Hatching Technique

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23
Q

will give and accurate and reliable
result.

A

egg hatch assay

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24
Q

All liquid medium commonly used for the isolation and maintenance
of Entamoeba histolytica and Balantidium coli

A

BALAMUTH’S MONOPHASIC MEDIUM

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25
Cyst to trophozoite of Entamoeba histolytica and Balantidium coli
BALAMUTH’S MONOPHASIC MEDIUM
26
Use of eggyolk
BALAMUTH’S MONOPHASIC MEDIUM
27
Entamoeba histolytica
Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw
28
Egg slant
Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw
29
Use of whole hen’s eggs
Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw
30
T. vaginalis
TRUSSEL AND JOHNSON’S MEDIUM
31
Entamoeba histolytica Giardia lamblia
AXENIC CULTURE METHOD
32
Monophasic clear liquid for rapid growth of dense populations of amoeba,
Axenic Culture Method
33
This medium is highly specific for E. histolytica , which grows luxuriantly on this medium.
Cleveland & Collier’s
34
Proteose peptone and infusion from liver provide amino acids and other nitrogenous substances that support growth of E. histolytica
Cleveland & Collier’s
35
In cleveland and collier’s what maintains the osmotic balance of the medium?
Sodium chloride
36
In cleveland and collier’s what acts as a phosphorus source
sodium a-glycerophosphate
37
Used to maintain bacteria-free cultures of the flagellate for teaching and research
SIMPLIFIED TRYPTICASE SERUM)
38
For Naegleria and Acanthamoeba from CSF or brain tissue
CULBERTSON'S MEDIUM
39
Simplest method for culturing Naegleria and acanthamoeba
WILLAERT'S MEDIUM
40
Uses non nutrient agar
WILLAERT'S MEDIUM
41
Seeded with heat killed or live bacteria usually E. coli
Willaert’s medium
42
Best medium
Novy, MacNeal and Nicole (NNN) Diphasic
43
CULTURE METHOD FOR LEISHMANIA & TRYPANOSOMES that use of sterile defibrinated rabbit blood
NOVY, MACNEAL AND NICOLE (NNN) DIPHASIC
44
Originial medium of NNN?
sterile defibrinated rabbit blood
45
Modified NNN
Schneider's insect medium with 10% fetal calf serum
46
Days of NNN?
10-21days
47
For Trypanosoma cruzi and Toxoplasma
Cellular media
48
Modification of the Moguchi-Battistini medium for culturing Bartonella bacilliformis
WEINMAN'S MEDIUM
49
Medium for Trypanosoma gambiense
WEINMAN'S MEDIUM
50
Cultivation of African Trypanosomes
TOBIE'S DIPHASIC MEDIUM
51
Positive stool samples are mixed with moistened soil of granulated charcoal and them larvae is harvested by Bornhermann procedure
Coproculture
52
Positive stool sample is applied to the filter paper? Filter paper is placed into a test tube with 7 mL of boiled or distilled water
HARADA-MORI CULTURE method
53
In harada mori hookworm filariform moves?
Downward
54
In harada mori strongyloides largae moves?
Upward
55
Uses uretral ang vaginal exudates as specimen
Simplified trypticase serum
56
Other intestinal amoebae do not grow readily on this medium
Cleveland and collier’s
57
Cleveland and collier’s technique of overlaying the medium with
Fresh sterile horse serum saline
58
as been an essential component of numerous immunological and biochemical studies
Axenic culture method
59
as been an essential component of numerous immunological and biochemical studies
Axenic culture method
60
Monophasic clear liquid for rapid growth of dense populations of amebae
Axenic culture media