Tech Flashcards
Cryptosporidium
SUGAR FLOTATION TECHNIQUE
We can isolate here the rounded oocysts and crescent
sporozoites
SUGAR FLOTATION TECHNIQUE/
uses sat. NaCl solution- 40g NaCl + 100 mL dist. H2O
WILLIE’S BRINE
4 consecutive negative results
CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD
Collection: morning before the patient washes or defecates
CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD
Enterobius vermicularis and Taenia
CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD
Estimates worm burden
EGG COUNT TECHNIQUE
✓ Determines the degree of infection (light, moderate or heavy)
Egg Count Technique –
For recovery of hookworms, Ascaris and Trichuris
Egg Count Technique –
1.5 mg feces (667) = epg
2 mg feces (500) = epg
DIRECT SMEAR EGG COUNT
-0.1 N NaOH
STOLL’S EGG COUNTING TECHNIQUE DILUTION EGG
COUNT
-KTS FOR SCHISTOSOMES
THICK SMEAR EGG COUNT
FOR
SCHISTOSOMES
DILUTION-FILTRATION EGG COUNT
The counts can be reported as eggs per direct smears
DIRECT SMEAR EGG COUNT
Dilution egg count is by?
By Stoll and Hausheer
The Kato cellophane covered thick smear is modified by
Martin and Beaver
For simultaneous qualitative and quantitative diagnosis of the common intestinal nematode infections
THICK SMEAR EGG COUNT
For routine quantitative diagnosis of Schistosoma mansoni and Schistosoma japanicum infections.
THICK SMEAR EGG COUNT
DILUTION-FILTRATION EGG COUNT is by?
Bell
can be concentrated filtering a suspension
of feces through filter paper on which the eggs are stained and counted.
Schistosome eggs- DILUTION-FILTRATION EGG COUNT
is a laboratory tool used to determine a given parasite’s resistance to extant drug therapy.
Egg hatching assay
Fresh eggs are incubated from the parasite of interest and serial
dilutions of the drug of interest are applied
Egg Hatching Technique
will give and accurate and reliable
result.
egg hatch assay
All liquid medium commonly used for the isolation and maintenance
of Entamoeba histolytica and Balantidium coli
BALAMUTH’S MONOPHASIC MEDIUM