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PARASIT > Tech > Flashcards

Tech Flashcards

1
Q

Cryptosporidium

A

SUGAR FLOTATION TECHNIQUE

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2
Q

We can isolate here the rounded oocysts and crescent
sporozoites

A

SUGAR FLOTATION TECHNIQUE/

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3
Q

uses sat. NaCl solution- 40g NaCl + 100 mL dist. H2O

A

WILLIE’S BRINE

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4
Q

4 consecutive negative results

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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5
Q

Collection: morning before the patient washes or defecates

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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6
Q

Enterobius vermicularis and Taenia

A

CELLULOSE TAPE PREPARATION/GRAHAM SCOTCH TAPE METHOD

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7
Q

Estimates worm burden

A

EGG COUNT TECHNIQUE

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8
Q

✓ Determines the degree of infection (light, moderate or heavy)

A

Egg Count Technique –

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9
Q

For recovery of hookworms, Ascaris and Trichuris

A

Egg Count Technique –

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10
Q

1.5 mg feces (667) = epg
2 mg feces (500) = epg

A

DIRECT SMEAR EGG COUNT

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11
Q

-0.1 N NaOH

A

STOLL’S EGG COUNTING TECHNIQUE DILUTION EGG
COUNT

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12
Q

-KTS FOR SCHISTOSOMES

A

THICK SMEAR EGG COUNT

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13
Q

FOR
SCHISTOSOMES

A

DILUTION-FILTRATION EGG COUNT

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14
Q

The counts can be reported as eggs per direct smears

A

DIRECT SMEAR EGG COUNT

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15
Q

Dilution egg count is by?

A

By Stoll and Hausheer

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16
Q

The Kato cellophane covered thick smear is modified by

A

Martin and Beaver

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17
Q

For simultaneous qualitative and quantitative diagnosis of the common intestinal nematode infections

A

THICK SMEAR EGG COUNT

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18
Q

For routine quantitative diagnosis of Schistosoma mansoni and Schistosoma japanicum infections.

A

THICK SMEAR EGG COUNT

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19
Q

DILUTION-FILTRATION EGG COUNT is by?

A

Bell

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20
Q

can be concentrated filtering a suspension
of feces through filter paper on which the eggs are stained and counted.

A

Schistosome eggs- DILUTION-FILTRATION EGG COUNT

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21
Q

is a laboratory tool used to determine a given parasite’s resistance to extant drug therapy.

A

Egg hatching assay

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22
Q

Fresh eggs are incubated from the parasite of interest and serial
dilutions of the drug of interest are applied

A

Egg Hatching Technique

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23
Q

will give and accurate and reliable
result.

A

egg hatch assay

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24
Q

All liquid medium commonly used for the isolation and maintenance
of Entamoeba histolytica and Balantidium coli

A

BALAMUTH’S MONOPHASIC MEDIUM

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25
Q

Cyst to trophozoite of Entamoeba histolytica and Balantidium coli

A

BALAMUTH’S MONOPHASIC MEDIUM

26
Q

Use of eggyolk

A

BALAMUTH’S MONOPHASIC MEDIUM

27
Q

Entamoeba histolytica

A

Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw

28
Q

Egg slant

A

Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw

29
Q

Use of whole hen’s eggs

A

Boeck & Drbohlav’s Diphasic as Modified by Dobell and Laidlaw

30
Q

T. vaginalis

A

TRUSSEL AND JOHNSON’S MEDIUM

31
Q

Entamoeba histolytica
Giardia lamblia

A

AXENIC CULTURE METHOD

32
Q

Monophasic clear liquid for rapid growth of dense populations of
amoeba,

A

Axenic Culture Method

33
Q

This medium is highly specific for E. histolytica , which grows luxuriantly
on this medium.

A

Cleveland & Collier’s

34
Q

Proteose peptone and infusion from liver provide amino acids and
other nitrogenous substances that support growth of E. histolytica

A

Cleveland & Collier’s

35
Q

In cleveland and collier’s what maintains the osmotic balance of the medium?

A

Sodium chloride

36
Q

In cleveland and collier’s what acts as a phosphorus source

A

sodium a-glycerophosphate

37
Q

Used to maintain bacteria-free cultures of the flagellate for
teaching and research

A

SIMPLIFIED TRYPTICASE SERUM)

38
Q

For Naegleria and Acanthamoeba from CSF or brain tissue

A

CULBERTSON’S MEDIUM

39
Q

Simplest method for culturing Naegleria and acanthamoeba

A

WILLAERT’S MEDIUM

40
Q

Uses non nutrient agar

A

WILLAERT’S MEDIUM

41
Q

Seeded with heat killed or live bacteria usually E. coli

A

Willaert’s medium

42
Q

Best medium

A

Novy, MacNeal and Nicole (NNN) Diphasic

43
Q

CULTURE METHOD FOR LEISHMANIA & TRYPANOSOMES that use of sterile defibrinated rabbit blood

A

NOVY, MACNEAL AND NICOLE (NNN) DIPHASIC

44
Q

Originial medium of NNN?

A

sterile defibrinated rabbit blood

45
Q

Modified NNN

A

Schneider’s insect medium with 10% fetal calf serum

46
Q

Days of NNN?

47
Q

For Trypanosoma cruzi and Toxoplasma

A

Cellular media

48
Q

Modification of the Moguchi-Battistini medium for culturing
Bartonella bacilliformis

A

WEINMAN’S MEDIUM

49
Q

Medium for Trypanosoma gambiense

A

WEINMAN’S MEDIUM

50
Q

Cultivation of African Trypanosomes

A

TOBIE’S DIPHASIC MEDIUM

51
Q

Positive stool samples are mixed with moistened soil of granulated charcoal and them larvae is harvested by Bornhermann procedure

A

Coproculture

52
Q

Positive stool sample is applied to the filter paper? Filter paper is placed into a test tube with 7 mL of boiled or
distilled water

A

HARADA-MORI CULTURE method

53
Q

In harada mori hookworm filariform moves?

54
Q

In harada mori strongyloides largae moves?

55
Q

Uses uretral ang vaginal exudates as specimen

A

Simplified trypticase serum

56
Q

Other intestinal amoebae do not grow readily on this
medium

A

Cleveland and collier’s

57
Q

Cleveland and collier’s technique of overlaying the medium with

A

Fresh sterile horse serum saline

58
Q

as been an essential component of numerous
immunological and biochemical studies

A

Axenic culture method

59
Q

as been an essential component of numerous
immunological and biochemical studies

A

Axenic culture method

60
Q

Monophasic clear liquid for rapid growth of dense populations of
amebae

A

Axenic culture media

PARASIT (29 decks)

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