synthetic biology Flashcards
what is one of the most advanced piece of engineering
a plant
which is the plant so advanced
self repairing, self replicating, high adaptable
however..
we don’t understand some parts like we do an iphone
definition
the application of engineering principles in order to design and build ew biological parts, denies and integrated biological systems
SynBio is interdisciplnary
bio, physics, mats, comp sciences
when did SynBio start
2000- first synthetic circuits- toggle switch and repressor
- auto regulatory negative feedback circuit
richard eynaman
what i cannot create, i cannot understand
central focma
replication of DNAvia DNA polymerase. RNA polymerase produces RNA. which a ribosome translates into proteins
biological constraints which present challenges and stop biology being engineerable
cell growth/ death, crosstalk, mutation, evolution, chemical diffusion, underlining biological processes that interfere, butterfly effect
to get around the unengineerable nature of biology
1) design
2) modelling
3) characterisation
4) abstraction
synthetic biology parts
promoter, cds, ribosome entry site, terminator, operator, insulator, ribonuclease site, ma stability, protease site, protease site, protein stability element, origin of replication, primer binding site, restriction site, blunt restriction site, sticky restriction site, assembly scar, signature, user defined
what is importnant
consistent building block
enabling technolgies
DNA sequencing , online databases, dna synthesis, dna assembly, lab automation
dna synthesising has
got much cheaper over th years, the amount of info from dna sequencing has also increased
get huge amount of inform from
low computing imput
logic gates
sets inputs and outputs, all us to compute, can be produced in cell
dna synthesising in modern times
send off and results come back within the week, instead of having to produce a vector, ligate, transform and screening of produced colony
basic process of synbio
design, build, test, learn
circuit of design/build/test/learn
has to be done 6/7 times until we have a fully engineer able biological system
which part is key
the learning
abstraction
1) DNA
2) Parts
3) devices
4) circuits
5) systems
6) multicellular
application of SynBio e.g. microbial cell factors
Artemisinin- precursors for antimalarial drug- can now brow in yeast
application of SynBio e.g. biosensors
pika and ebola- paper based- toehold switches
