key biochemical tests Flashcards
casein hydrolysis
detects presence of caseinase
caseinase
s an enzyme that hydrolyses the milk protein casein
what does casein hydrolysis differentiate between
aerobic actinomycetes- based on casein utilisation
does streptomycin or nocadia use casein
streptomycin
catalase test
detects catalase
catalase
converts H2O2 to H2O and O2
which bacteria does the catalase test differentiation
staph and bacillus from clostridium
coagulas test
detects presence of coagulase- causes plasma to clot
esculin hydrolysis
tests for cleavage of glycoside
what is the esculin test used to differentiate betwen
staph, strept and others
B galactosidase
demonstates the presence of an enzyme that cleaves lactose to glucose and galactose
b-galactosidase separates
enteric bacteria e.g. citrobacter and salmonella
B-galactosidae reaction
hydrolyses lactose to glucose and galactose