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General Microbiology > Review of Microscopy > Flashcards

Review of Microscopy Flashcards

1
Q

functions to magnify the image of the objects that cannot be seen by the naked eye

A

microscope

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2
Q

parts of the microscope based on function (4)

A

mechanical part
adjustable part
illuminating part
magnifying part

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3
Q

illuminating parts include (3)

A

mirror/light source
condenser
iris diaphragm

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4
Q

identify parts of microscope

A

+1

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5
Q

magnifying parts (5)

A

ocular/eye piece
scanner
LPO
HPO
OIO

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6
Q

magnification of eye piece

A

10x

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7
Q

magnification of scanner

A

4x

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8
Q

magnification of LPO

A

10x

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9
Q

magnification of HPO

A

40x

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10
Q

magnification of OIO

A

100x

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11
Q

an optical microscope that uses light rays to produce a dark image against a bright background

A

bright field microscope/
compound light microscope

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12
Q

Ability of the microscope to enlarge an image

A

magnification

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13
Q

It depends on the degree of curvature of the glass lens

A

magnification

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14
Q

Highly/lowly curved lens will increase the magnification

A

highly

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15
Q

Magnification depends on the degree of ____of the glass lens.

A

curvature

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16
Q

relationship of glass lens curvature and magnification

A

directly proportional

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17
Q

Ability of the lens to distinguish two points as clear and as distinct.

A

resolution

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18
Q

resolution depends on what (2)

A

wavelength of light (λ)
numerical aperture (NA)

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19
Q

wavelength symbol

A

λ

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20
Q

relationship of wavelength of light (λ) to Resolution

A

inversely proportional

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21
Q

refers to the light-gathering ability of the lens

A

numerical aperture

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22
Q

expressing mathematically the solid cone of light delivered to the specimen.

A

numerical aperture

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23
Q

relationship of resolution with NA

A

directly proportional

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24
Q

numerical aperture and resolution for LPO

A

NA = 0.25
Resolution = 0.9µm

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25
numerical aperture and resolution for HPO
NA = 0.65 Resolution = 0.35 µm
26
numerical aperture and resolution for OIO
NA = 1.25 Resolution= 0.18µm
27
the amount light bends
refractive index
28
if the RI of two materials are the same, would there be bending of light?
no
29
the amount of distance required between the objective lens and the top of your object.
focal length
30
the higher the objective magnification, the shorter/longer the focal length generally is.
shorter
31
A technique used to measure the dimensions of the microorganisms.
micrometry
32
two kinds of micrometer
ocular stage
33
measures the dimensions of the microorganisms
ocular micrometer
34
calibrates the ocular micrometer
stage micrometer
35
conversion factor of mm = um
1 mm = 1,000 µm
36
a stage micrometer has the measure of
1mm
37
how many stage unit are in a stage micrometer
100
38
measurement of 1 stage unit
0.01mm or 10um
39
formula used to compute the calibration factor for 1 ocular unit
(X) (OU) = (Y) (SU)
40
X in (X) (OU) = (Y) (SU)
divisions of ocular micrometer (OM) subtended by divisions of stage micrometer
41
OU in (X) (OU) = (Y) (SU)
ocular unit
42
Y in (X) (OU) = (Y) (SU)
divisions of stage micrometer (SM) subtended by divisions of ocular micrometer
43
SU in (X) (OU) = (Y) (SU)
stage unit
44
stage unit value
0.01mm or 10um
45
thus 1 OU =
(Y)(SU)/(X) or (SM div.)(10um)/(OM div)
46
Value of 1 OU in microns for scanner
62.5um
47
Value of 1 OU in microns for LPO
50.0
48
Value of 1 OU in microns for HPO
12.5
49
Value of 1 OU in microns for OIO
5 um
50
s an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image
phase contrast microscope
51
main foundation of the microscope
base
52
connects the arm with the base (some do not have the pillar)
pillar
53
part of the microscope where we usually carry the microscope
arm
54
structure that would connect the draw tube, and revolving nosepiece
body tube
55
structure where eyepiece/ocular are placed
draw tube
56
square platform where you place the specimen
stage
57
initial focusing knob, manipulated to adjust the actual gap between the specimen and objective (scanner and LPO)
coarse adjustment knob
58
initial focusing knob, manipulated to adjust the actual gap between the specimen and objective (scanner and LPO)
coarse adjustment knob
59
also known as final focusing, manipulated when the specimen is already visible (HPO and OIO)
fine adjustment knob
60
holds the specimen in place
stage clip
61
adjusted for you to move the stage clip
stage clip knob
62
hold the objective so you can shift from one objective to another
revolving nose piece
63
part of microscope that is usually the one that provides the light
illuminating part
64
reflects light from the environment
mirror/light source
65
structure above the light source, gather the light from the light source
condenser
66
control the light coming to the specimen
iris diaphragm
67
part of the microscope that is utilized to magnify the specimen
magnifying parts
68
– 10x, where we look at the specimen being observed
ocular/eye piece
69
what oil is used in oil immersion
cedar oil
70
why is oil used in OIO
minimize refractive index
71
– utilize light to observe specimen
bright field microscopy
72
entire circle seen when you look in the microscope
field of view
73
what kind of image is created when looking at a microscope
enlarged virtual inverted
74
color of scanner
red
75
color of lpo
yellow
76
color of hpo
blue
77
color of oio
white
78
100 SU is equal to what mm
1mm
79
1mm is equal to what microns
1000 microns
80
thus, 1 SU is equal to what microns
10 microns
81
memorize table for 10x10 and 20x20
+1

General Microbiology (25 decks)

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