Review of Microscopy Flashcards
functions to magnify the image of the objects that cannot be seen by the naked eye
microscope
parts of the microscope based on function (4)
mechanical part
adjustable part
illuminating part
magnifying part
illuminating parts include (3)
mirror/light source
condenser
iris diaphragm
identify parts of microscope
+1
magnifying parts (5)
ocular/eye piece
scanner
LPO
HPO
OIO
magnification of eye piece
10x
magnification of scanner
4x
magnification of LPO
10x
magnification of HPO
40x
magnification of OIO
100x
an optical microscope that uses light rays to produce a dark image against a bright background
bright field microscope/
compound light microscope
Ability of the microscope to enlarge an image
magnification
It depends on the degree of curvature of the glass lens
magnification
Highly/lowly curved lens will increase the magnification
highly
Magnification depends on the degree of ____of the glass lens.
curvature
relationship of glass lens curvature and magnification
directly proportional
Ability of the lens to distinguish two points as clear and as distinct.
resolution
resolution depends on what (2)
wavelength of light (λ)
numerical aperture (NA)
wavelength symbol
λ
relationship of wavelength of light (λ) to Resolution
inversely proportional
refers to the light-gathering ability of the lens
numerical aperture
expressing mathematically the solid cone of light delivered to the specimen.
numerical aperture
relationship of resolution with NA
directly proportional
numerical aperture and resolution for LPO
NA = 0.25
Resolution = 0.9µm
numerical aperture and resolution for HPO
NA = 0.65
Resolution = 0.35 µm
numerical aperture and resolution for OIO
NA = 1.25
Resolution= 0.18µm
the amount light bends
refractive index
if the RI of two materials are the same, would there be bending of light?
no
the amount of distance required between the objective lens and the top of your object.
focal length
the higher the objective magnification, the shorter/longer the focal length generally is.
shorter
A technique used to measure the dimensions of the microorganisms.
micrometry
two kinds of micrometer
ocular
stage