Isolation Techniques Flashcards
Bacterial suspension consisting of a single species.
pure culture
mixture of cells
macroscopic or microscopic
microscopic
separation of cells by spreading or dilution in agar medium
macroscopic or microscopic
microscopic
growth increases the number of cells
macroscopic or microscopic
microscopic
microbes become visible as isolated colonies containing millions of cells
macroscopic or microscopic
macroscopic
Reducing the number of bacterial cells from the previous streaks.
streak plating
how many quadrants streak plating
4
have heavy confluent growth
area 1
have less dense growth
area 2
have weak growth
area 3
what isolated single colonies
area 4
Spreading 0.1 ml sample of suspension in an agar plate
spread plating
example of selective media
eosin methylene blue agar
has green sheen to indicate e.coli
eosin methylene blue agar
quantify how many bacteria is present in the sample
spread plating
1m = __ microleter
1000
pour plating of what method
8 method
spread plating procedure
sample is pipetted onto surface of agar plate (___ what measurement)
0.1ml or 100ul
what is used to spread sample over the surface of agar using sterile
glass spreader
Used to measure microbial load
spread plating result
Pouring 1.0 ml sample of suspension in an empty plate and then addition of melted agar on to
pour plating
Used to measure microbial load
pour plating
is a common technique used to culture obligate and anaerobic bacteria by isolating microbial colonies.
pour plate method
method of pour-plate method
how much sample is used in pour plate method
1.0 ml or 1000ul
subculturing technique step 1
Pick out a well-isolated colony with an inoculating needle.
subculturing technique step 2
Transfer the organism from the Petri plate to a tube of nutrient broth or a slant of nutrient agar.
subculturing technique step 3
Incubate for 24 hours.
subculturing technique step 4
A stained slide of the culture can be made to determine if a pure culture has been achieved.
is a step-wise series of dilutions, where the dilution factor stays the same for each step.
serial dilution
amount of saline solution
9.0ml
serial dilution factor for spread and pour plating
10^-4
label for 30-1000 colonies,
TNTC (too numerous to count)
label for less than 30 colonies
TFTC
suitable number of colonies
30-300
