Isolation Techniques

Question 1

Bacterial suspension consisting of a single species.

Answer 1

pure culture

Question 2

mixture of cells

macroscopic or microscopic

Answer 2

microscopic

Question 3

separation of cells by spreading or dilution in agar medium

macroscopic or microscopic

Answer 3

microscopic

Question 4

growth increases the number of cells

macroscopic or microscopic

Answer 4

microscopic

Question 5

microbes become visible as isolated colonies containing millions of cells

macroscopic or microscopic

Answer 5

macroscopic

Question 6

Reducing the number of bacterial cells from the previous streaks.

Answer 6

streak plating

Question 7

how many quadrants streak plating

Answer 7

4

Question 8

have heavy confluent growth

Answer 8

area 1

Question 9

have less dense growth

Answer 9

area 2

Question 10

have weak growth

Answer 10

area 3

Question 11

what isolated single colonies

Answer 11

area 4

Question 12

Spreading 0.1 ml sample of suspension in an agar plate

Answer 12

spread plating

Question 13

example of selective media

Answer 13

eosin methylene blue agar

Question 14

has green sheen to indicate e.coli

Answer 14

eosin methylene blue agar

Question 15

quantify how many bacteria is present in the sample

Answer 15

spread plating

Question 16

1m = __ microleter

Answer 16

1000

Question 17

pour plating of what method

Answer 17

8 method

Question 18

spread plating procedure

sample is pipetted onto surface of agar plate (___ what measurement)

Answer 18

0.1ml or 100ul

Question 19

what is used to spread sample over the surface of agar using sterile

Answer 19

glass spreader

Question 20

Used to measure microbial load

Answer 20

spread plating result

Question 21

Pouring 1.0 ml sample of suspension in an empty plate and then addition of melted agar on to

Answer 21

pour plating

Question 22

Used to measure microbial load

Answer 22

pour plating

Question 23

is a common technique used to culture obligate and anaerobic bacteria by isolating microbial colonies.

Answer 23

pour plate method

Question 24

method of pour-plate method

how much sample is used in pour plate method

Answer 24

1.0 ml or 1000ul

Question 25

subculturing technique step 1

Answer 25

Pick out a well-isolated colony with an inoculating needle.

Question 26

subculturing technique step 2

Answer 26

Transfer the organism from the Petri plate to a tube of nutrient broth or a slant of nutrient agar.

Question 27

subculturing technique step 3

Answer 27

Incubate for 24 hours.

Question 28

subculturing technique step 4

Answer 28

A stained slide of the culture can be made to determine if a pure culture has been achieved.

Question 29

is a step-wise series of dilutions, where the dilution factor stays the same for each step.

Answer 29

serial dilution

Question 30

amount of saline solution

Answer 30

9.0ml

Question 31

serial dilution factor for spread and pour plating

Answer 31

10^-4

Question 32

label for 30-1000 colonies,

Answer 32

TNTC (too numerous to count)

Question 33

label for less than 30 colonies

Answer 33

TFTC

Question 34

suitable number of colonies

Answer 34

30-300