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General Microbiology > Isolation Techniques > Flashcards

Isolation Techniques Flashcards

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Biology 101 flashcards
1
Q

Bacterial suspension consisting of a single species.

A

pure culture

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2
Q

mixture of cells

macroscopic or microscopic

A

microscopic

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3
Q

separation of cells by spreading or dilution in agar medium

macroscopic or microscopic

A

microscopic

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4
Q

growth increases the number of cells

macroscopic or microscopic

A

microscopic

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5
Q

microbes become visible as isolated colonies containing millions of cells

macroscopic or microscopic

A

macroscopic

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6
Q

Reducing the number of bacterial cells from the previous streaks.

A

streak plating

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7
Q

how many quadrants streak plating

A

4

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8
Q

have heavy confluent growth

A

area 1

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9
Q

have less dense growth

A

area 2

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10
Q

have weak growth

A

area 3

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11
Q

what isolated single colonies

A

area 4

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12
Q

Spreading 0.1 ml sample of suspension in an agar plate

A

spread plating

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13
Q

example of selective media

A

eosin methylene blue agar

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14
Q

has green sheen to indicate e.coli

A

eosin methylene blue agar

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15
Q

quantify how many bacteria is present in the sample

A

spread plating

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16
Q

1m = __ microleter

A

1000

17
Q

pour plating of what method

A

8 method

18
Q

spread plating procedure

sample is pipetted onto surface of agar plate (___ what measurement)

A

0.1ml or 100ul

19
Q

what is used to spread sample over the surface of agar using sterile

A

glass spreader

20
Q

Used to measure microbial load

A

spread plating result

21
Q

Pouring 1.0 ml sample of suspension in an empty plate and then addition of melted agar on to

A

pour plating

22
Q

Used to measure microbial load

A

pour plating

23
Q

is a common technique used to culture obligate and anaerobic bacteria by isolating microbial colonies.

A

pour plate method

24
Q

method of pour-plate method

how much sample is used in pour plate method

A

1.0 ml or 1000ul

25
Q

subculturing technique step 1

A

Pick out a well-isolated colony with an inoculating needle.

26
Q

subculturing technique step 2

A

Transfer the organism from the Petri plate to a tube of nutrient broth or a slant of nutrient agar.

27
Q

subculturing technique step 3

A

Incubate for 24 hours.

28
Q

subculturing technique step 4

A

A stained slide of the culture can be made to determine if a pure culture has been achieved.

29
Q

is a step-wise series of dilutions, where the dilution factor stays the same for each step.

A

serial dilution

30
Q

amount of saline solution

A

9.0ml

31
Q

serial dilution factor for spread and pour plating

A

10^-4

32
Q

label for 30-1000 colonies,

A

TNTC (too numerous to count)

33
Q

label for less than 30 colonies

A

TFTC

34
Q

suitable number of colonies

A

30-300

General Microbiology (25 decks)

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