Microbial Motility

Question 1

The ability of an organism to move by itself.

Answer 1

motility

Question 2

the ability to orientate along certain chemical gradients

Answer 2

chemotaxis

Question 3

have different locomotory organelles.
Cilia, flagella, pseudopods

Answer 3

eukaryotic cells

Question 4

– the motile forms have unique flagella (propeller-type), axial filament or by special fibrils that allow a gliding motion.

Answer 4

prokaryotic cells

Question 5

Two (2) types of movement(as observed in samples under the microscope

Answer 5

brownian movement
true motility

Question 6

first observed in 1827 by the English botanist Robert Brown.

Answer 6

brownian movement

Question 7

first observed brownian movement

Answer 7

Robert Brown

Question 8

Motility due to random molecular bombardment of tiny bacterial cells by the molecules of the solvent

Answer 8

brownian movement

Question 9

motility due to special locomotory structures

Answer 9

true motility

Question 10

self propelled motility

Answer 10

true motility

Question 11

movement in a purposeful way, though may change direction frequently.

Answer 11

true motility

Question 12

Three (3) techniques in observing microbial motility

Answer 12

wet mount
hanging drop
culture method

Question 13

Slide methods (for non-pathogenic bacteria) (2)

Answer 13

wet mount
hanging drop

Question 14

method for pathogenic bateria

Answer 14

culture mount/soft agar stabbing

Question 15

wet mount technique

Answer 15

place two loopfuls of inoculum (15-20ul)
cover with cover slip
observe under LPO

Question 16

magnification for wet mount technique

Answer 16

LPO

Question 17

hanging drop technique

Answer 17

add small amt of vaseline on each corner of cover glass

two loopfuls of organisms in the center of cover glass

place depression slide against vaseline and quickly inverted

observe under OIO

Question 18

magnification for drop technique

Answer 18

OIO

Question 19

culture technique (stabbing)

Answer 19

dip the inoculating needle onto the suspension
inoculate the motility medium
incubate at 37oC for 48 hours

Question 20

duration for stab technique

Answer 20

37oC at 48 hours

Question 21

 Crucial for microorganisms to survive in their environment

Answer 21

motility

Question 22

ability to orientate along certain chemical gradients

Answer 22

chemotaxis

Question 23

occurs if the movement is toward a higher concentration of the chemical in question

Answer 23

positive chemotaxis

Question 24

signals include nutrition/favorable conditions

Answer 24

chemoattractant

Question 25

a chemical substance that provokes chemotaxis, esp one that causes a bacterium to move in the direction in which its concentration is increasing.

Answer 25

chemoattractant

Question 26

the movement of organisms away from chemicals

Answer 26

negative chemotaxis

Question 27

chemical that make the organism go away

Answer 27

chemorepellent

Question 28

hair-like projection in the cell that enable it to move (ex. Paramecium)

Answer 28

cilia

Question 29

whip-like structure but have tubulin in eukaryotes

Answer 29

flagella

Question 30

false feet (seen in amoeba), a temporary arm-like projection of a eukaryotic cell membrane that is emerged in the direction of movement.

Answer 30

pseudopods

Question 31

• the motile forms have a unique flagella (propeller-type), axial filament or by special fibrils that allow a gliding motion

Answer 31

prokaryotic cells

Question 32

 pins, creating forward movement by a corkscrew shaped filament

Answer 32

prokaryotic flagella