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General Microbiology > Bacteriological Analysis of Water > Flashcards

Bacteriological Analysis of Water Flashcards

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1
Q

are a group of closely related, mostly harmless bacteria that live in soil and water as well as the gut of animals.

A

coliforms

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2
Q

is a hygienic indicator and high level of coliform counts generally indicates unsanitary condition or poor hygiene practices during or after food production

A

coliform count

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3
Q

These are organisms present in the environment and in the feces of all warm-blooded animals and humans

A

coliform bacteria

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4
Q

will not likely cause illness

A

coliform bacteria

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5
Q

coliform bacteria is

gram positive/negative

A

gram negative

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6
Q

oxygen requirement of coliform bacteria

A

facultative anaerobic

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7
Q

shape of coliform bacteria

A

rod shape

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8
Q

does coliform bacteria ferment lactose

A

yes

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9
Q

coliform ferments lactose to produce what

A

acid and gas

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10
Q

coliform bacteria ferments lactose to produce acid and gas within what variables

A

48 hours at 35oC

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11
Q

used as an indicator of sanitary quality of water or as a general indicator of sanitary condition in the food-processing environment.

A

detection of coliforms

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12
Q

three groups of coliform bacteria

A

total coliform
fecal coliform
E.coli (O157:H7)

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13
Q

indicates environmental contaminaton

A

total coliform

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14
Q

fecal coliform + e. coli =

A

fecal contamination

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15
Q

Are commonly found in the environment (soil or vegetation) and are generally harmless.

A

total coliform bacteria

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16
Q

If only __ ____ bacteria are detected in drinking water, the source is probably environmental

A

total coliform

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17
Q

____ contamination is not likely if total coliform is detected

A

fecal

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18
Q

Are a sub-group of total coliform bacteria.

A

fecal coliform bacteria

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19
Q

They appear in great quantities in the intestines and feces of people and animals

A

fecal coliform bacteria

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20
Q

its presence often indicates recent fecal contamination, meaning that there is a greater risk that pathogens are present than if only total coliform bacteria is detected

A

fecal coliform bacteria

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21
Q

It is a sub-group of the fecal coliform group.

A

e. coli

22
Q

Most___ bacteria are harmless and are found in great quantities in the intestines of people and warm-blooded animals

A

e.. coli

23
Q

straincauses a severe intestinal infection in humans

A

O157:H7

24
Q

The presence of___ in a drinking water sample almost always indicates recent fecal contamination, meaning there is a greater risk that pathogens are present.

A

E. coli

25
Q

___or treating contaminated drinking water with a ___destroys all forms ofE. coli, includingO157:H7.

A

boiling
disinfectant

26
Q

It is a method for estimating the number of bacteria in a food or water sample.

A

most probable number method

27
Q

In this technique, replicate portions of the original sample are cultured to determine the presence or absence of microorganisms in each portion.

A

most probable method

28
Q

three phases of MPN

A

presumptive
confirmatory
completed

29
Q

Almost all the methods used to detect E. coli

A

MPN

30
Q

are enumeration methods that are based on lactose fermentation

A

total coliform or fecal coliform

31
Q

It is used for detection and estimation of coliform in water sample.

A

presumptive test

32
Q

broth in presumptive test

A

lactose containing broth medium

33
Q

positive result of turbidity

A

turbidity of media and gas formation

34
Q

equipment used for presumptive test

A

Durham’s tube

35
Q

MPN table will be used to estimate the most probable number of viable cell.

A

presumptive test

36
Q

Confirmed test is done to determine that the coliforms are of fecal origin or not.

A

confirmed test

37
Q

Also determine if the isolated bacteria is E. coli or not.

A

confirmed test

38
Q

are inoculated in Brilliant Green Lactose bile Broth (BGLBB) broth.

A

positive presumptive test

39
Q

broth for confirmed test

A

Brilliant Green Lactose Bile Broth (BGLBB)

40
Q

Positive BGLBB samples are inoculated in selective media like

A

eosin methylene blue agar

41
Q

indicates the presence of E. coli.

A

green metallic sheen colonies

42
Q

is performed on all samples showing a positive result in the confirmed test.

A

completed test

43
Q

is e coli spore forming

A

no

44
Q

The formation of gas in the fermentation tube and the presence of gram-negative, non-spore-forming, rod-shaped bacteria in the agar culture may be considered a satisfactorily ___ test

A

completed

45
Q

1 mpn = __ cfu = ___ viable cell

A

1 cfu
1 viable cell

46
Q

presuumptive test complete equipment

A

injective tube
durham tube

47
Q

presumptive test kind of strength

A

5 double strength
10 single strength

48
Q

doube the concentration of lactose broth used to test for coliform organisms in water

A

double strength

49
Q

how many water sample is placed in double strenth

A

10 ml

50
Q

how many water ml is placed in SS tubes (both amount)

A

1 ml
0.1 ml

51
Q

to confirm strain of e. coli, it has to undergo

A

antigen-antibody test

General Microbiology (20 decks)

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