Microbiolab Supplemental Flashcards
⚫Also an indicator of spoilage
microbial counts
how many ul of inoculum is placed in a hemocytometer
10 ul
how many ul of inoculum is placed in pour plating
1 ml
how many ul is placed in spread plating
0.1 ml
direct count formula
(5)(10)(1000)(DF)
cfu/ml formula
number of colonies * DF/volume plated
⚫Anaerobic to Facultative bacteria will grow.
what kind of bacteria lcount
pour plating
⚫Usually lower count than Spread Plating because of the
hot medium poured into the bacterial suspension
pour plating
can be used to measure
aerobic to facultative anaerobes
spread plating
measures anaerobic to facultative anaerobe
pour plating
⚫It is possible that ___ plating method will give a lower
count because the melted medium can kill the cells.
pour
cell wall synthesis
cycloserine
vancomycin
bacitracin
penicillin
cephalosporins
monobactams
carbapenems
dna gyrase
nalidixic acid
ciproflaxin
novobiocin
quinolones include
nalidixic acid
ciproflaxin
rna elongation
actinomycin
cycloserine
vancomycin
bacitracin
penicillin
cephalosporins
monobactams
carbapenems
what do they inhibit
cell wall synthesis
nalidixic acid
ciproflaxin
novobiocin
what do they inhibit
dna gyrase
actinomycin
what do they inhibit
RNA elongaton
inhibit dna-directed rna polymerase
rifampin
streptovaricins
rifampin
streptovaricins
what do they inhibit
DNA-directed rna polymerase
50s inhibitors
erythromycin
chloramphenicol
clindamycin
lincomycin
erythromycin
chloramphenicol
clindamycin
lincomycin
what do they inhibit
50s inhibitors (protein synthesis)
30s inhibitors
Tetracyclines
Spectinomycin
Streptomycin
Gentamicin
Amikacin
Kanamycin
Nitrofurans
Tetracyclines
Spectinomycin
Streptomycin
Gentamicin
Amikacin
Kanamycin
Nitrofurans
what do they inhibit
30s inhibitors