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General Microbiology > Culture Media Preparation > Flashcards

Culture Media Preparation Flashcards

1
Q

– can also be prepared for fungi and bacteria

A

culture media

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2
Q

– virus can be grown in this

A

living cells

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3
Q
  • the survival of microorganisms depend on
A

 available nutrients
 favorable growth environment

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4
Q

nutrient preparations that are used for growing microorganisms

A

media or culture media

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5
Q

microbes that grow and multiply or on a culture medium
* three physical forms of media

A

culture

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6
Q
  • three physical forms of media
A

liquid or broth media
semisolid media
solid media

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7
Q

ex. Nutrient broth, tryptic soy broth, brain-heart infusion broth

A

liquid or broth media

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8
Q

– have agar (ex. Motility medium)

A

semisolid media

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9
Q

have agar (ex. Nutrient agar, blood agar)

A

solid media

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10
Q

complex carbohydrate (polysaccharide) extracted from seaweed

A

agar

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11
Q

 a solidifying agent

A

agar

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12
Q

 solid agar melts about

A

100 degrees c

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13
Q

 liquid agar solidifies at about

A

42 degrees c

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14
Q

 while in the liquefied state, semi solid and solid media can be poured into either a test tube or petri dish/plate

A

agar

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15
Q

type of medium (5)

A

broth
agar slant
agar deep
agar stab
agar plate

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16
Q

autoclaving container for

broth
agar slant
agar deep
agar stab
agar plate

A

culture tube (broth - agar stab)
~200ml culture bottle or EM flask (agar plate)

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17
Q
  • types of Culture Media (2)
A

 chemically defined or synthetic
 Chemically complex or non-synthetic

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18
Q

composed of known amounts of pure chemicals

A

chemically defined or synthetic

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19
Q

 used in culturing autotrophic microorganisms such as algae or non-fastidious heterotrophs

A

chemically defined or synthetic

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20
Q

composed of complex materials that are rich in vitamins and nutrients
 beef extract, yeast extract, and peptones

A

chemically complex or non synthetic

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21
Q
  • culture media preparation
A
  1. calculation and weighing
  2. mixing with water and heating
  3. sterilization
  4. fine-tuning (supplements and pH)
  5. dispensing
  6. packing and storage
  7. quantity control
  8. use of dispensed culture media
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22
Q

temperature higher than 50oC poured in media can cause this

A

condensates

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23
Q

used to prevent buildup of condensation

A

inverted media

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24
Q
  • Sterilization of media and materials for microbial culture
A

 Culture dishes
 Test tubes
 Flasks
 Pipettes
 Transfer loops
 media

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25
using an oven, this kind of sterilization is carried out at 150oC to 180oC for 1 to 4 hours
dry heat sterilization
26
 Can be used for glassware, fixed oils, thermostable powders
dry heat sterilization
27
 sterilization using this requires higher temperatures and longer
dry heat sterilization
28
 heat transfer is slow, small volumes of oil and thin layers should be used
dry heat sterilization
29
in the presence of moisture, microorganisms are destroyed at a lower temperature than in dry heat
moist heat sterilizaton
30
 autoclave is using heat + pressure
moist heat sterilization
31
 180 degrees at 150 psi  121.0 °C / 249.8 °F at 15 lbs / square inch (psi) for 15 minutes  Method of choice when product can withstand treatment (heat stable)
dry heat sterilizaton
32
* Steam sterilization applications
 Solutions sealed in containers ampules, vials  Media  Bulk Solutions  Glass wares (ex. tubes with media)  Surgical Dressing  Instruments
33
* Advantages of steam sterilization:
 Rapid, Inexpensive, Effective, Large volumes
34
* Disadvantages of steam sterilization:
 Cannot be used for oily preparation (oil base ointment)  Cannot be used for moisture sensitive preparations
35
where culture medium is sterilized in
autoclave
36
varies from original value when subjected to heat sterilization (can increase/decrease_
pH of media
37
most bacteria grow best at this pH
pH 6.5-7.5
38
– pH should be adjusted after this
sterilization
39
very little grow at this pH
pH 4
40
may grow in pH as low as 1
acidophiles
41
- must be adjusted after sterilization if the pH of medium strays too far from neutral.
pH
42
* Preparation of media from commercial dehydrated products
 Each bottle of dehydrated medium has instructions for preparation on its label  Nutrient agar 23g/1000ml
43
formula to find the g needed
=(how many g (how many mL))/1000mL
44
n order to make 400 mL of medium with a concentration of 15 g/L, how much medium powder would you weigh in grams
6g
45
* In order to make 650 mL of medium with a concentration of 20 g/L, how much medium powder would you weigh in grams?
13g
46
* To fill up 10 agar plates that contains 20 mL of medium with a concentration of 36 g/L, how much medium powder would you weigh in grams?
7.2g
47
* Preparation of Nutrient Broth (NB), a chemically complex medium
 Prepare 300 ml of NB  Weigh appropriate NB powder and dissolve in 300 ml dH2O  Check initial pH of medium  Divide contents into 3 flasks  = 100 ml in each
48
* Sterilization of media:
 1st flask – in autoclave (121⁰C, for 15 mins.)  2nd flask – in oven (170 ⁰C, for 1 hr.)  3rd flask – no sterilization
49
* Preparation of Glucose Minimal Salth Broth (GMSB)
 Weigh media powder appropriate for 300 ml.  Combine media and dH2O in flask.  Check initial pH of medium  Divide contents into 3 flasks  = 100 ml in each  Sterilize. - autoclave
50
retain coloration and structure of bacteria during staining
mordant (gram's iodine)
51
helps in the removal of primary stains from bacteria
decolorizer
52
escherichia coli shape gram positive/negative
Gram-negative, rod-shaped
53
Bacillus subtilis gram positive/negative
gram positive, rod-shaped
54
Proteus vulgaris gram/positive negative
gram-negative, rod-shaped
55
Serratia marceschens gram/positive negative
rod-shaped, Gram-negative
56
Pseudomonas aeruginosa gram/positive negative
rod-shaped Gram-negative rod
57
Staphylococcus aureus gram positive/negative
Gram-positive, spherical or cocci-shaped
58
dye for positive staining
methylene blue
59
is a simple tool used primarily by microbiologists to take and transfer a small sample (inoculum) of a microorganism culture
inoculation loop
60
view experiment results on gc
+1
61
20 petri dishes will be used that will have 20mL NA/plate 28.0g/1000mL compute for the grams
(28g)(20x20)/1000mL 11.2g
62
Normal Saline Solution abbreviation
SNSS
63
20 test tubes will be filled with 10 mL per test tubes How much NaCl will be used to make 0.9% SNSS soln?
wt% = g solutes/ml of soln *100 0.009(250) = g g= 2.25g

General Microbiology (25 decks)

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