Antimicrobial Sensitivity Testing

Question 1

Substances produced by microorganisms that kill or inhibit the growth of other microorganisms

Answer 1

antibiotics

Question 2

Should have high selective toxicity

Answer 2

antibiotics

Question 3

Must undergo sensitivity testing

Answer 3

antibiotics

Question 4

Must kill or inhibit the microbial pathogen without damaging the host cell.

Answer 4

selective toxicity

Question 6

is the regulation of gene expression in response to fluctuations in cell-population density

Answer 6

quorum sensing

Question 7

this kind of bacteria produce and release chemical signal molecules called autoinducers that increase in concentration as a function of cell density.

Answer 7

quorum sensing bacteria

Question 8

MRSA meaning

Answer 8

Methicillin Resistant Staphylococcus Aureus

Question 9

antibiotic attacking cell wall synthesis (6 - CVBPCMC)

Answer 9

Cycloserine
Vancomycin
Bacitracin
Penicillins
Cephalosporins
Monobactams
Carbapenems

Question 10

antibiotic targeting DNA gyrase (3)

Answer 10

Nalidixic acid
Ciprofloxacin
Novobiocin

Question 11

group of antibiotic targeting DNA gyrase

Answer 11

Quinolones

Question 12

targets transcription (RNA elongation)

Answer 12

Actinomycin

Question 13

targets DNA-directed RNA polymerase (2)

Answer 13

Rifampin
Streptovaricins

Question 14

targets protein synthesis (50S inhibitors) (4)

Answer 14

Erythromycin
Chloramphenicol
Clindamycin
Lincomycin

Question 15

targets protein synthesis (30S Inhibitors) (6)

Answer 15

Tetracyclines
Spectinomyces
Streptomycin
Gentamicin
Kanamycin
Amikacin
Nitrofurans

Question 16

targets protein synthesis (tRNA) (2)

Answer 16

Mupirocin
Puromycin

Question 17

targets folic acid metabolism (2)

Answer 17

Trimethoprim
Sulfonamides

Question 18

targets cytoplasmic membrane structure (2)

Answer 18

Polymyxin
Daptomycin

Question 19

Used to determine the MIC (minimum inhibitory concentration) and MLC (minimum lethal concentration)

Answer 19

dilution susceptibility test

Question 20

tests to determine the level of antimicrobial activity

Answer 20

Dilution Suceptibility Test
Kirby-Bauer Method (Disk Diffusion Test)

Question 21

other name for Disk Diffusion Test

Answer 21

Kirby-Bauer Method

Question 22

Can test the resistance or sensitivity of an organism to an array of antimicrobial agents.

Answer 22

Kirby-Bauer Method

Question 23

is the lowest concentration of a chemical, usually a drug, which prevents visible in vitro growth of bacteria or fungi.

Answer 23

minimum inhibitory concentration

Question 24

When Barium is completely suspended, the optical density of the suspension is 0.5

Answer 24

McFarlan 0.5 turbidity standard

Question 25

approximate number of Mcfarland units in the inoculum with 0.5 MFU

Answer 25

1.5x10^8 cfu/ml

Question 26

MFU means

Answer 26

McFarland Standard

Question 27

Medium used for standardized antimicrobial disk diffusion susceptibility testing.

Answer 27

Mueller-Hinton Agar

Question 28

MHA is the best medium for routine susceptibility tests because (3)

Answer 28

good reproducibility low in sulfonamide, trimethoprim, tetracyline inhibitors gives satisfactory growth of most bacterial pathogens

Question 29

area where mcirobes cannot grow

Answer 29

zone of inhibition

Question 30

MHA is low in (3) what inhibitors

Answer 30

sulfonamide trimethoprim tetracycline

Question 31

draw the streaking pattern for the inoculum

Answer 31

+1

Question 32

is a circular area around the spot of the antibiotic in which the bacteria colonies do not grow

Answer 32

zone of inhibition

Question 33

can be used to measure the susceptibility of the bacteria to wards the antibiotic.

Answer 33

zone of inhibition

Question 34

see the zone size interpretive chart for Kirby-Bauer Test

Answer 34

+1

Question 35

Cycloserine Vancomycin Bacitracin Penicillin Cephalosporin Monobactams Carbapenems targets what

Answer 35

Cell wall synthesis

Question 36

Nalidixic acid Ciproflaxin Novobiocin targets what

Answer 36

DNA gyrase

Question 37

Actinomycin targets what

Answer 37

RNA elongation

Question 38

Rifampin Streptovaricin targets what

Answer 38

DNA-directed RNA polymerase

Question 39

Erythromycin is also known as

Answer 39

macrolides

Question 40

Erythromycin Chloramphenicol Clindamycin Lincomycin targets what

Answer 40

protein synthesis (50s inhibitors)

Question 41

Tetracycline Spectinomyces Streptomyces Gentamicin Kanamycin Amikacin Nitrofurans targets what

Answer 41

protein synthesis (30s inhibitors)

Question 42

Mupirocin Puromycin targets what

Answer 42

Protein synthesi (tRNA)

Question 43

Trimethrophin Sulfonamides targets what

Answer 43

folic acid metabolism

Question 44

Polymyxin Daptomycin targets what

Answer 44

cytoplasmic membrane structure