Pentose phosphate pathway Flashcards
The three metabolic pathways of glucose are?
Oxidation via glycolysis to yield pyruvate, ATP & NADH
Formation of polysaccharides
Oxidation via PPP to yield ribose-5-phosphate & NADPH for reductive biosynthesis
An alternative metabolic pathway for glucose oxidation in which no ATP is generated is called?
Pentose phosphate pathway (PPP)
PPP is also known as?
Hexose monophosphate shunt (HMP Shunt)
6-Phosphogluconate pathway
Four properties of PPP are?
More complex pathway than glycolysis
More anabolic in nature
Takes place in the cytosol
Concerned with the biosynthesis of NADPH & pentoses
Four tissues that are highly active in HMP shunt are?
Adrenal gland
Liver
Testes
Lactating mammary gland
The two products of PPP are?
NADPH
Pentose
Four importance of NADPH are?
Biosynthesis of fatty acids and steroids
detoxification of drugs
Phagocytosis requires NADPH
NADPH keeps the Fe2+ iron of haemoglobin in a reduced state
Two importance of Pentoses are?
Use in the synthesis of nucleic acids and many nucleotides
Produces carbohydrates for cell wall synthesis for some growing organisms
Nucleic acid examples are?
DNA
RNA
Nucleotide examples are?
ATP
NAD+
coA
HMP Shunt sequence of reactions are divided into?
Two phases
The first phase of the HMP shunt is?
Oxidative phase(non-reversible)
The oxidative phase has how many ___reactions
3
In the first reaction, __1__ catalyzes the conversion of Glucose-6-phosphate into __2__
- Glucose-6-phosphate dehydrogenase(G6PD)
2. 6-phosphogluconolactone
The two products of the first reaction are?
6-phosphogluconolactone and NADPH
In the second reaction, 6-phosphogluconolactone is hydrolyzed into?
6-phospho-D-gluconate
Gluconolactonase
In the third reaction, oxidative decarboxylation of 6-phospho-D-gluconate is done to produce?
Ribulose-5-phosphate
NADPH
(6 phosphogluconate dehydrogenase)
PPP is most active in?
Cells where large amounts of lipids are synthesized (liver, adipose tissue, mammary glands)
Cells that are at high risk for oxidative damage (RBC)
Under the oxidative phase of PPP, Glucose-6-phosphate undergoes __1__ & _2__ to give a pentose, and D-ribulose-5-phosphate
Dehydrogenation
Decarboxylation
Non-oxidative phase has ___ reactions
2
In the first reaction, ribulose-5-phosphate is converted to ribose-5-phosphate by?
Ribulose-5-phosphate isomerase
Ribulose-5-phosphate may also be converted to xylulose-5-phosphate by?
Ribulose-5-phosphate epimerase (main reaction)
__ &__ catalyze the interconversions of trioses, Pentoses & hexoses
Transketolase
Transaldolase
A TPP requiring enzyme that transfers two carbon units from a ketose to an aldose is called?
Transketolase