PARVOVIRIDAE

Question 1

Subfamilies

Answer 1

(Parvo=Small)
1. Parvovirinae and

2. Densovirinae
   (infect arthropods = unimportant-crop protection)

• Genera:
• Densovirus
• Brevivirus

Question 2

Genera Parvoviridae

Answer 2

Parvovirus
Amdovirus
Bocavirus
Erthythrovirus
Dependovirus

Question 3

Morphology

Answer 3

Non-Enveloped
Icosahedral capsid
ssDNA (linear)

Question 4

Resistance

Answer 4

Very strong
Enviromentally stable

Resistant to:

• Acids and Bases
• Lipid solvents
• Heat of 60°C for 60 mins
• Inactivated by:
  Formalin, and
  other oxidising agents

Question 5

Replication

Answer 5

• Baltimore II
• Replication only in the nuclei of host cells
• Cellular polymerase converts:
  ssDNA -> dsDNA.
• Virions are released by cell lysis.

Question 6

Biological properties

Answer 6

Stenoxenous
Haemadsorbtion
(Complementation-> use: Aden or herpes infected cells)

Question 7

Antigenic properties

Answer 7

Very good antigens with long immunity.
Have cross reactions

Related viruses:
- Mink enteritis canine parvovirus 2

Question 8

Diagnosis

Answer 8

Isolation in dividing cells - intranuclear ICB
- HA
- IF
- AGID
= Less sensitive
- HAI
- VN
= Serology type

Question 9

Parvovirus

Answer 9

1. Porcine parvovirus (SMEDI):
   - Stillbirths and embryonic death + infertility
2. Canine parvovirus 2:
   - Highly contagious enteritis disease with: *vomiting,
   *dysentery and
   * immunosuppression. also
   * myocarditis in pups
   (inflammation of GI trackt) + Myocarditis
3. Feline Planleukopenia:
   - Highly contagious enteric disease with:
   * Depression,
   * vomiting and
   * diarrhoea.
   (Stemcell/WBC - immunity suppressed)
4. Mink Enteritis:
   Generalized disease of mink kittens.
   - Similar to Feline Planleukopenia.
5. Chicken parvovirus

Question 10

Amdovirus

Answer 10

1. Aleutian mink disease(AMD).
   - Persistant viraemia,
   - Plasmacytosis,
   - Hypergamma-globulinaemia,
   - Hemorrhage and
   - Nephritis.

Imunocomplex disease - Nephritis

Question 11

Bocavirus

Answer 11

1. Bovine parvovirus.
   - Sporadic diarrhoea in Calves.
2. Canine minute virus (CPV-1).

Question 12

Erthythrovirus

Answer 12

1. B19 virus: HUMANS
   - Anaemia
   - Human transient aplastic crisis and
   - Foetal wastage.
2. Simian Erythrosis

Question 13

Dependovirus

Answer 13

1. Adeno associated viruses - need other viruses: eg. Herpes
2. Goose parvovirus aka
   - DERZSY´s disease:
   
   • Respiratory disease and (4weeks old)
   • Enteritis.
3. Muscovy duck parvovirus.