Other Blood Groups Flashcards
Diego system chromosome
17
red cell membrane anion exchanger
band 3
what antigens are located on Band 3, red cell membrane anion exchanger?
Diego
Diego antibodies are what antibody class?
IgG
does anti-Dia cause HDFN?
yes
does anti-Dia bind complement?
yes
Diego A is a ___ frequency antigen
low
Diego B is a ___ frequency antigen
high
does anti-Dib cause HDFN?
yes
Wright A is a ___ frequency antigen
low
Wright B is a ___ frequency antigen
high
does anti-Wra cause HDFN?
yes
Wright antibodies are what antibody class?
IgG
which Wright antibody is a common autoantibody?
anti-Wrb
effect of enzymes on Diego system antigens
resistant
Yt system is on chromosome ___
7
previous name for Yt antigens
Cartwright
where are Yt system antigens located
AChE (achetylcholeinsterase)
which Yt antigen is high frequency
Yta
which Yt antigen is low frequency
Ytb
effect of ficin, papain, DTT, pronase on Yt antigens
destroyed
effect of trypsin and acid on Yt antigens
resistant
Yt(a+b-) phenotype frequency
92% all populations
Yt(a-b+) phenotype frequency
rare
Yt(a+b+) phenotype frequency
7% all populations
Yt antibody class
IgG
are Yt antibodies clinically significant
no
which Yt antibody has caused rare incidence of acute and delayed HTR
anti-Yta
Xg blood group antigen
Xga
Xg antigen on chromosome ___
X
Xga phenotype frequency
89% females
66% males
Xg antigen expression on cord cells?
weak
effect of ficin and papain on Xg antigens
destroyed
effect of DTT on Xg antigens
resistant
which “other” blood group antigen is a useful genetic marker
Xg
Xga antibody class
IgG
which “other” blood group system antigen may appear as mixed field due to low density of antigen sites
anti-Xga
does anti-Xga cause HTR or HDFN?
no
which blood group antigen system is found on erythrocyte membrane-associated protein
Scianna
low frequency Scianna antigens
Sc2 and Sc4
high frequency Scianna antigens
Sc1 and Sc3
effect of enzymes on Scianna antigens
resistant
Scianna null phenotype
Sc:1-2-3-
what population is Scianna null phenotype seen in?
Marshall Islands, Papua New Guinea
Scianna antibody class
IgG
are Scianna antigens clinically significant
no
what antibody is produced by people with rare Scianna null phenotype
anti-Sc3
Dombrock antigens
Doa Dob Gya Hy Joa
are Dombrock antigens high or low frequency
high
effect of enzymes on Dombrock antigens
resistant
Dombrock antibody class
IgG
are Dombrock antibodies clinically significant?
yes
can Dombrock antibodies cause HDFN?
yes
what population develops anti-Gya
Dombrock null phenotype
where are Colton system antigens found
aquaphorin-1, water transporter
which Colton antigen is high frequency
Coa
which Colton antigen is low frequency
Cob
antigen frequency of Co3
100% all populations
Colton antibody class
IgG
do Colton antibodies cause HDFN
yes
what cells do anti-Co3 react with
all red cells except rare Colton null phenotype
Colton antigens are found on what body parts
blood cells and kidneys
which LW system antigen is high frequency
LWa
which LW system antigen is low frequency
LWb
LW(a+b-) phenotype frequency
97% all populations
LW(a-b+) phenotype frequency
rare all populations
LW(a+b+) phenotype frequency
3% most populations
6% Finns
anti-LWa may be mistaken for which antibody?
anti-D
autoanti-LW common in what disease association?
warm autoimmune hemolytic anemia
what effect does D negative antigen status have on LW expression?
D neg adults = decreased LW expression
is LW expressed on cord cells?
yes
where do Gerbich system antigens reside
GPC and GPD structures
Yus phenotype:
Ge:-2, 3, 4
Gerbich phenotype:
Ge:-2,-3,4
Leach phenotype:
Ge:-2,-3,-4
what is Gerbich null phenotype
Leach phenotype Ge:-2,-3.-4
what causes Gerbich null phenotype?Kell
deficiency in GPC and GPD
what antigen expression is affected by Gerbich nul phenotype?
Kell
Gerbich antibody class
IgG, few IgM
are Gerbich antibodies clinically significant
no
Cromer system chromosome
1
Cromer system antigens located on:
complement regulator protein
effect of ficin, papain, glycine-acid EDT on Cromer antigens
resistant
effect of DTT treatment on Cromer antigens
weakened
effect of alpha chymotrypsin on Cromer antigens
destroyed
null Cromer phenotype
Inab
Cromer antigens expressed on what cells?
RBCs, WBCs, platelets
Cromer antibody class
IgG
are Cromer antibodies clinically significant
no
what inhibits Cromer antibodies?
serum or concentrated urine
how are Cromer antibodies removed from serum?
platelet concentrates
what antibody is made by Cromer null phenotype?
anti-IFC
which Indian antigen is low frequency?
Ina
which Indian antibody is high frequency?
Inb
what antigens function as lymphocyte homing receptors?
Indian
what antigens function as CD44 adhesion molecules?
Indian
what antigen is microbial receptor for polio virus?
Indian
Indian antibody class
IgM
are Indian antibodies clinically significant?
no
Vel antibody class
IgM
is Vel expressed on cord cells
weakly
does anti-Vel cause HTR
ues
what cell is AnWj expressed o?
Lu(a-b-)
what antigen is a microbial receptor for Haemophilus influenzae?
AnWj
what antigens are part of 901 high incidence series
Vel
AnWj
Sda
what antibody demonstrates with refractile mixed-field agglutination at AHG?
anti-Sda
how is anti-Sda neutralized
urine from Sda positive individuals
is Sda present on cord cells?
no
does Sda cause HTR
no
why can anti-Sda be seen in Sda positive prenatal patients
antigen suppression during pregnancy
where are HLA antigens found
all nucleated cells
what are HLA antigens on red cells sometimes called?
Bennett-Goodspeed (Bg)
what HLA class are Bg antigens
HLA class I
Bga is what HLA antigen
HLA-B7
Bgb is what HLA antigen
HLA-B17 (B57 or B58)
Hbc is what HLA antigen
HLA-A28 (A68 or A69)
how can HLA antigens be removed from red cells
chloroquine or EDTA/glycine-HCl
what effect do enzymes, AET, and DTT have on HLA antigens
resistant
are HLA antibodies clinically significant
no in transfusion, yes in transplant and platelet refractoriness
in what blood bank practice are HLA antibodies imporant
platelet refractoriness
what does low avidity mean in HTLA
affinity of antibody to antigen
what titer characteristic identifies HTLA antibodies
weak reactions with undiluted serum with titrations showing same weak reactions out to high dilutions
HTLA antibody class
IgG
list “HTLA” antibodies
Chido (Cha) Rodgers (Rga) Knops (Kna) McCoy (McCa) SIa York (Yka) Cost-Sterling (Csa) John-Milton-Hagen (JMH)
are Chido/Rodgers system antigens high or low frequency
high
antigens found on 4th component (C4d) molecule of complement
Chido/Rodgers
are Chido/Rodgers antigens present on cord cells?
weak or absent
effect of enzymes on Chido/Rodgers antigens
destroyed
effect of DTT on Chido/Rodgers antigens
resistant
inhibition of anti-Ch1 and anti-Rg1
soluble plasma antigen or complement found in fresh human serum
what enhances Chido/Rodgers antibody reactivity?
testing against C4d-coated red cells
Knops system chromosome
1
antigens in Knops system
Kna (Knops)
McCa (McCoy)
SIa
Yka (York)
what antigen group is found on complement-regulatory glycoprotein complement receptor-1 (CR-1)
Knops
antigen system depressed in lupus, AIDS, malignancies
Knops
effect of papain and ficin on Knops antigens
weakened
effect of trypsin on Knops antigens
trypsin
which Knops antibody is more common in black populations
anti-McCa
which Knops antibody is more common in white populations
anti-Kna
is JMH antigen high or low frequency
high
effect of enzymes and DTT on JMH
destroyed
is JMH expressed on cord cells
weakly or no
JMH antibody class
IgG
is anti-JMH clinically significant
no
auto-anti-JMH often found in what patient population
elderly
Cost system serologically like what other blood group system
Knops
high frequency antigen of Cost system
Csa
effect of enzymes on Cost antigen
resistant
Cost antibody class
IgG
is anti-Csa clinically significant
no
Cost null phenotype
Cs(a-b-) Kn(a-b-) McC(a-) SI(a-) Yk(a-)
