Duffy and Kidd Flashcards
Duffy system chromosome
1
gene responsible for FY antigen
ACKr1 (atypical chemokine receptor 1)
Fya amino acid
glycine
Fyb amino acid
asparagine
Duffy antigens on cord cells?
yes
effect of ficin and papain on Fya and Fyb antigens
destroyed
effect of thiol reagents on Fya and Fyb antigens
not affected
Duffy antigen present when Fya or Fyb present
Fy3, Fy5, Fy6
effect of enzymes, pronase, DTT, acid on Fy3
resistant
Duffy antigen that requires normal Rh for expression
Fy5
Duffy antigen not expressed on Rh null cells
Fy5
effect of enzymes, pronase, DTT, acid on Fy5
resistant
cells negative to which Duffy antigen are resistant to P. knowlesi and P. vivax but not P. falciparum?
Fy6
effect on ficin/papain, pronase on Fy6
destroyed
effect of trypsin, DTT, acid on Fy6
resistant
gene mutation where Duffy antigens are not expressed on red cells but are expressed on tissues
Fy(a-b-)
Duffy phenotype that provides resistance to P. vivax and P. knowlesi
Fy(a-b-)
Duffy phenotype that can make anti-Fy5
Fy(a-b-)
does Fy(a-b-) phenotype make anti-Fya or anti-Fyb?
no
true null Duffy phenotype where Duffy antigens are not expressed on red cells or tissues
FyFy
can FyFy phenotype make anti-Fya and anti-Fyb?
yes
Fy(a+b-) phenotype frequency
20% white
10% black
Fy(a-b+) phenotype frequency
32% white
20% black
Fy(a+b+) phenotype frequency
48% white
2% black
Fy(a-b-) phenotype frequency
very rare white
68% black
Duffy antibody class
IgG
do Duffy antibodies bind complement?
no
how much more common is anti-Fya than anti-Fyb?
20x
Duffy antibody usually made secondary to other antibodies
anti-Fyb
Duffy antibody found only in immunized Fy(a-b-) white individuals
anti-Fy3
how to distinguish anti-Fy3 from combination anti-Fya anti-Fyb?
enzymes will not destroy reactions of anti-Fy3
Duffy antibody found in blacks with Fy(a-b-) phenotype
anti-Fy5
Kidd system gene
JK
JK gene located on chromosome ___
18
Kidd system antigens located on…
red blood cells and kidney tissue
red cell antigens carried by human erythroid urea transport protein HUT11
Jka and Jkb
antigens associated with urea transport mechanism
Kidd
red cell antigens that reside as antigen clusters on red cell surface
Kidd
Kidd antigens on cord cells?
yes
effect of enzymes on Kidd antigens
enhanced
effect of DTT, AET, chloroquinine diphosphate on Kidd antigens
resistant
Jk(a+b-) phenotype frequency
28% white
57% black
Jk(a-b+) phenotype frequency
23% white
9% black
Jk(a+b+) phenotype frequency
49% white
34% black
Jk(a-b-) phenotype frequency
rare in all populations
Jka antigen frequency
77% white
91% black
Jkb antigen frequency
73% white
43% black
Jk3 antigen frequency
100%
rare homozygous JkJk silent gene phenotype
true null Jk(a-b-)
dominant inhibitor suppressor gene Kidd phenotype
In(Jk)
test for Jk(a-b-) phenotype
2M urea lysis
effect of 2M urea on Jk(a-b-) cells
resist lysis, shrink and shrivel
effect of 2M urea on normal Kidd antigen cells
swell and lyse
Kidd antibody class
IgG
do Kidd antibodies bind complement?
yes
IgG antibody that looks like inseparable anti-JkaJkb
anti-Jk3
anti-Jk3 reacts with which cells?
all cells except Jk(a-b-)
