ABO/H/Secretor/Lewis Flashcards
ABO antigens detected on embryos at ___ age
5-6 weeks
ABO antigens on chromosome __
9
H antigen substrate
fucose
formation of A, B, and H antigens results from interactions of genes on 3 separate loci: __, __, ___
ABO, Hh, Se
H gene
FUT1
Secretor gene (Se)
FUT2
fuctosyltransferase
H and Se genes located on chromosome ___
19
active secretor gene
Se
inactive secretor gene
se
Se/se gene frequency
80% secretors (SeSe or Sese)
20% non-secretors (sese)
inheritance of Se gene results in ____ added to ____ chains resulting in type 1 H antigen substances in secretions
fucose/type1
inheritance of H gene results in ___ added to ____ chains resulting in type 2 H antigens on red blood cells
fucose/type 2
H gene transferase
alpha-2-L-fucosyltransferase
A gene transferase
alpha-3-acetryl-galactosaminyl-transferase
B gene transferase
alpha-3-D-galactosyltransferase
O gene transferase
none; gene is non-functional
oligosaccharide chain linkage in secretions
beta 1-3
oligosaccharide chain linkage on red cells
beta 1-4
A1 subgroup frequency
80% of A population
A2 subgroup frequency
20% of A population
anti-A1 lectin
Dolichos biflorus
expected reaction of A1 cells with Dolichos biflorus
positive
Ax subgroup presentation
no reaction with anti-A
weak reaction with anti-A,B
may produce anti-A1
Am subgroup presentation
no reactions with anti-A or anti-A,B
Aend subgroup presentation
weak reactions with anti-A and anti-A,B
Ael subgroup presentation
no reactions with anti-A or anti-A,B but eluates contain anti-A
Ay subgroup presentation
no reactions with anti-A, anti-A1, or anti-A,B
B3 subgroup presentation
+/mf anti-B
+/mf anti-A,B
no anti-B in serum
secretes H
Bx subgroup presentation
0/+ anti-B
0/++ anti-A,B
no anti-B in serum
secretes B and H
Bm subgroup presentation
+/++ anti-B
+/++ anti-A,B
anti-B in serum
secretes B and H
arises from inheritance of nonfunctional FUT1 gene, recessive genotype hh, and nonfunctional Se gene
Bombay phenotype
Bombay phenotype types as ___
O
Bombay phenotype rare disease association
Leukocyte Adhesion Deficiency 2 (LAD2) – lack of fucose
Bombay phenotype antibodies in serum
anti-A
anti-B
anti-H
Bombay phenotype secretor status
non-secretor
Para-Bombay secretor status
secretor
Para-Bombay phenotype presentation
H-deficient secretors
homozygous for nonfunctional H gene but inherit at least 1 normal functional secretor gene
para-Bombay phenotype serum reactions
anti-H and/or anti-IH
lectin
plant seed extract that agglutinates human red cells with high degree of specificity
anti-H lectin
Ulex europaeus
isoagglutinins
term reserved for expected antibodies in ABO system
alloantibodies
individual that has the antibody will not have the corresponding antigen
ABO antibody type
IgM
anti-A,B
formed by group O individuals and present in fairly high concentrations
anti-A,B antibody class
IgG and IgM
ABO antibody most likely in ABO HDN
anti-A,B
anti-A,B HDN
group O moms make IgG anti-A,B when carrying group A or B baby
autoantibody produced by group A1 and A1B
anti-H
H antigen expression on blood types
O > Ax > A2 > B > A2B > A1 > A1B > Oh
blood group with most H antigen expression
O
(normal) blood group with least H antigen expression
A1B
