Blood Components Flashcards
transport temp of whole blood
1-10C
storage temp of whole blood
1-6C
expiration of CPDA whole blood
35 days
CPDA shelf life
35 days
CPDA with additive solution shelf life
42 days
open system packed cell expiration
24 hours
CPDA unit hematocrit requirement
<80%
additive solution unit hematocrit
~55-65%
indications for washed packed cells
history of allergic trx rxn
IgA deficiency with anti-IgA
washed RBC expiration
24 hours
indications for leukocyte reduced RBCs
febrile trx rxn due to HLA antibodies
immunosuppressed/immunodeficient
prevent production of HLA antibodies in chronic trx
irradiated RBC shelf life
28 days or original shelf life (whichever comes first)
why are irradiated red cells given a shortened expiration date?
irradiation weakens RBC membrane, gives unacceptable 24 hour red cell survival after 28 days and increases supernatant potassium
liquid red cell storage temp
1-6C
liquid red cell transport temp
1-10C
how long after collection are liquid red cells stored?
within 24 hours
effects of increased blood storage temperature
accelerates metabolism
enzymes become active
bacterial growth
effects of decreased blood storage temp
nutrients not utilized
danger of accidental freezing/crystal formation
cells rupture
red cell storage lesion changes
dec plasma pH dec 2,3-DPG dec ATP dec dextrose dec nitric oxide inc plasma Hgb inc plasma potassium inc plasma ammonia
storage conditions including preservative solutions must provide for survival of red cells so that there are at least ___% viable cells at 24 hours post-transfusion
75
storage temp of frozen red cells
-80C or lower, -65C max
what cryoprotective agent is used in frozen red cells?
glycerol
shelf life of frozen red cells
10 years
when after collection can red cells by frozen?
within 6 days or after rejuvenation
effect of rejuvenation on red cell units older than 6 days to be frozen
restore 2,3-DPG to near normal
restore ATP to normal or higher
shelf life of thawed frozen red cells
24 hours
why are red cells from sickle trait donors not frozen?
cannot be deglycerolized, unit forms into gel-mass and hemolyzes during declygerolization
indications for frozen/deglycerolized red cells
store blood of donors with rare types
store autologous RBCs
IgA deficient with anti-IgA
severe allergic reactions
if declygerolized units are unused, when an they be refrozen?
within 20 hours of original thaw
what standard applies to rejuvenation of red cells?
5.7.4.4
red cell rejuvenation solution contains?
pyruvate
inosine
adenine
phosphate
purpose of red cell rejuvenation
restore 2,3-DPG and ATP levels
when can CPD or CPDA-1 red cells be rejuvenated?
after 14 days of liquid storage or up to 3 days past expiration date
what can be done to the product after red cell rejuvenation?
washed and transfused within 24 hours
glycerolized and frozen
when can AS-1 cells be rejuvenated?
anytime before expiration date
frozen storage expiration of rejuvenated, glycerolized AS-1 red cells
3 years
whole blood storage temp until platelets are removed
20-24C
centrifugation pattern for platelet preparation
light spin –> platelet rich plasma –> heavy spin, remove plasma
pH requirement for platelets
> 6.2
platelet rest time before storage
1 hour
minimum platelets per individual donor unit
5.5x10^10
platelet expiration if seal broken
4 hours
closed platelet pool expiration
5 days
open platelet pool expiration
4 hours
platelet storage lesion
dec ATP
dec ADP
dec pH
dec platelet glycoprotein
composition of fresh frozen plasma
all coagulation factors, including labile V and VIII, antithrombin, ADAMTS13
plasma frozen within ___ of whole blood collection
8 hours
FFP storage
<18C or colder
FFP transport media
dry ice
FFP frozen expiration
1 year
thawed FF expiration
24 hours (can be 5 days)
FFP expiration with broken seal
24 hours
what defines 24-hr plasma (PF24)
plasma frozen between 8-24 hours after collection
24-hour plasma has reduced levels of?
factor V and VIII
single donor plasma (liquid plasma)
plasma that has been separated from whole blood up to 5 days after whole blood expiration date
factors present in cryo
fibrinogen
VIII
vWF
XIII
main indication for cryo
fibrinogen replacement
when is cryo prepared?
anytime during 1 year storage of FFP
how is cryo prepared?
FFP placed in 1-6C and allowed to slowly thaw, precipitate forms –> cryo
units VIII per cryo bag
80
mg fibrinogen per cryo bag
150
cryo storage
<18C, optimal -30C or colder
frozen cryo expiration
1 year from original whole blood collection date
thawed cryo expiration
6 hours
thawed cryo storage temp
20-24C
open pooled cryo expiration
4 hours
indication for cryo-reduced plasma
TTP patients in plasma exchange therapy when non-responsive to albumin or FFP exchanges
max red cell loss per week during apheresis
25 mL
washed platelet shelf life
4 hours
cryoprotective agent in frozen platelets
DMSO (dimethyl sulfoxide)
shelf life of frozen platelets
2 years
volume reduced platelet shelf life
4 hours (washed)
donor Hct requirement for 2-unit red cell apheresis
> 40%
2-unit red cell apheresis donation interval
16 weeks
2-unit red cell apheresis male height/weight requirement
5ft1in
130 lb
2-unit red cell apheresis female donor requirement
5ft5in
150 lb
indication for plasma derived factor VIII
hemophilia A
contraindication for recombinant FVIII
von Willebrand’s disease
indication for factor XI
hemophilia B
hemophilia A with factor VIII inhibitors
use of 5% albumin
hypovolemic shock, burn patients, therapeutic apheresis
use of 25% albumin
cerebral edema
advantage of albumin
remains intravascular, increases colloid osmotic pressure, rapidly expands volume, pulls fluid from interstitium
temperature QC required on blood storage devices
must record continuously and be manually checked daily
manual reading every 4 hours is not continuous automated check
deviations recorded on chart with date and tech ID
products transported at 1-10C on wet ice
whole blood
red cells
thawed or liquid plasma
products transported frozen on dry ice
frozen red cells
frozen plasma
frozen cryo
products transported at 20-24C
platelets
granulocytes
thawed or liquid cryo
