MNS/Lutheran/Kell/Kx Flashcards
MNS system is on chromosome ___
4
M and N antigens located on what gene and glycoprotein?
GPYA
glycophorin A
U, S, s antigens located on what gene and glycoprotein?
GPYB
glycophorin B
what glycoproteins are used by Plasmodium falciparum as structures to enter red cells?
glycophorin A and glycophorin B
molecule that carries vast amounts of sialic acid that contributes to negative charge around red cell
glycophorin
enzyme effect on M and N antigens
destroyed due to cleavage of areas in amino acid chain
effect of chymotrypsin, DTT, and acid on M and N antigens
resistant
S and s expressed on cord cells?
yes
M and N expressed on cord cells?
yes
effect of chymotrypsin and pronase on S and s
destroyed
effect of trypsin, DTT, acid on S and s
resistant
MNS5 antigen
U
U expressed on cord cells?
yes
antigen frequency of U antigen
99% black
99.9% white
effect of enzymes on U
resistant
MNS system antigen carried on envelope of RBCs
En^a
effect of DTT and chymotrypsin on Ena
resistant
effect of ficin and trypsin on Ena
destroyed
MNS system antigen caused by silent mutation resulting in rearrangement of amino acids on GPA
Mg
MNS system antigen associated with weak U positive cells
Henshaw (He)
‘N’
N like antigen carried on GPB high prevalence (all cells except deficient of GPB)
MNS system antigen found in 7% of Chinese and 10% or greater in Thais
Mur
Mur is on which MNS system gene?
GPB
most common antibody seen after a-A and a-B in Chinese and Thai populations
anti-Mur
MNS system antigen on cells U- with weak expression of little s
Dantu
MNS system deletion or variant of GYPA resulting in no GPA, resulting in M-N- red cells
En(a-)
MNS system deletion of variant of GYPB resulting in no GPB
U negative
MNS deletion that provides protection against Plasmodium falciparum
U negative
MNS system true null phenotype
MkMk
MkMk null phenotype
M-N-En(a-)S-s-U-
MNS deletion phenotype with reduced levels of sialic acid on red cells
MkMk
anti-M antibody class
IgM
MN antibody that can sometimes be enhanced by lowering pH of serum to 6.5
anti-M
anti-M lectin
Iberis amara
anti-N antibody class
IgM
anti-N lectin
Vicia graminea
MN antibody historically seen in kidney dialysis patients due to residual formaldehyde
anti-Nf
anti-S antibody class
IgG
anti-s antibody class
IgG
anti-U antibody class
IgG
anti-U presentation on panels
combination of anti-S and anti-s
when is U antigen present?
if S or s is present
Lutheran system chromosome
19
are Lutheran antigens expressed on cord cells?
no
effect of trypsin, AET/DTT on Lutheran antigens
destroyed
effect of papain and ficin on Lutheran antigens
resistant or weakly affected
Lua antigen frequency
8% white
5% black
Lub antigen frequency
99.8%
which Lutheran antigen is low prevalence?
Lua
which Lutheran antigen is high prevalence?
Lub
majority of individuals are what Lutheran phenotype?
Lu(a-b+)
Lu3 antigen frequency
100%
Auberger antigens are in which system?
Lutheran
Aua antigen frequency
90%
Aub antigen frequency
50% white
68% black
autosomal amorphic recessive Lutheran phenotype
Lu null
only Lutheran phenotype to produce anti-Lutheran antibody
Lu null
autosomal dominant inhibitor gene Lutheran phenotype
In(Lu)
marked suppression of production of all Lutheran system antigens
In(Lu)
Lutheran phenotype that results in reduced expression of P1, I, AnWj, Indian, Knops, Cost, MER2
In(Lu)
X-linked recessive suppressor gene Lutheran phenotype
Lu mod
rare Lutheran phenotype carried on X chromosome
Lu mod
anti-Lua antibody class
IgM
Lutheran antibody that produces loose or stringy mixed-field agglutination
anti-Lua
anti-Lub antibody class
IgG
Lutheran antibody made by immunized individuals of rare recessive type Lu(a-b-)
anti-Lu3
XK gene is on which chromosome?
X
antigen of KX blood group system
Kx
gene of Kx blood group system
XK
antigen required for full expression of Kell system antigens
Kx
precursor for Kell antigens
Kx
KEL gene located on chromosome?
7
Kell system antigens expressed on cord cells?
yes
effect of DTT, ZZAP, 2-AET, EDTA/glycine-acid on Kell antigens
destroyed
effect of enzymes on Kell system antigens
resistant
___ gene encodes for Kell antigens inherited as a haplotype
KEL
normal Kell haplotype
k, Kpb, Jsb
number of mutations per Kell haplotype
one
K antigen frequency
9% white
2% black
25% Arab
k antigen frequency
> 99.9% all populations
Kpa antigen frequency
2-3% white
never seen in other populations
Kpb antigen frequency
99.9% all populations
Jsa antigen frequency
20% black
low frequency in other populations
Jsb antigen frequency
> 99.9%
KEL1 antigen
K
KEL2 antigen
k
KEL3 antigen
Kpa
KEL4 antigen
Kpb
KEL6 antigen
Jsa
KEL7 antigen
Jsb
Kell system antigen expressed 100% in all populations on all red cells except K0 phenotype
Ku
Kell system antigen expressed 100% in all populations on all red cells except McLeod phenotype and K0 phenotype
Km
inheritance of homozygous silent gene K0 (K0K0)
Kell null
Kell phenotype with weak expression of Kell antigens
Kmod
Kell phenotype caused by lack of or mutation in XK gene, lack of Kx antigen
McLeod
McLeod phenotype individuals make anti-___ when transfused
anti-KL
physiologic condition associated with McLeod phenotype
X-linked chronic granulomatous disease
Kell phenotype: no Kell antigens no Ku no Km increased Kx anti-Ku
Null (K0)
Kell phenotype:
marked reduction in Kell antigens
increase in Kx
anti-Ku like antibody
Kmod
Kell phenotype: reduction in Kell antigens but present no Km no Kx anti-KL
McLeod CGD
Kell phenotype: reduction in Kell antigens no Km no Kx anti-Km
McLeod non-CGD
Kell antibody class
IgG
do Kell antibodies bind complement?
no
3rd most potent antibody after ABO and Rh
Kell
