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Microbiology > Microbiology Exam #1 Lab Info > Flashcards

Microbiology Exam #1 Lab Info Flashcards

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Biology 101 flashcards
1
Q

describe the steps of a gram stain?

A
  1. flood slide with crystal violet for 60sec, then wash it off with water
  2. flood slide with gram’s iodine for 20sec, then wash it off
  3. decolorize carefully/indirectly
  4. flood slide with gram’s safranin for 60sec
  5. rinse off slide and dry with bibulous/blotting paper
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2
Q

which cell type remains purple in gram stain?

A

gram positive - crystal violet

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3
Q

which cell type is red in gram stain?

A

gram negative - safranin

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4
Q

gram positive or negative?

A

positive

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5
Q

gram postive or negative?

A

positive

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6
Q

gram positive or negative?

A

negative

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7
Q

what does a catalase test test for?

A

only organisms with a catalase enzyme (ex: Staphylococcus) will have a positive reaction when hydrogen peroxide added to sample

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8
Q

what does a positive catalase test look like?

A

bubbling once hydrogen peroxide added

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9
Q

what does a negative catalase test look like?

A

NO bubbling when hydrogen peroxide added

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10
Q

describe alpha hemolysis

A

partial, greenish halo around bacterial colonies
result of hydrogen peroxide produced by bacteria acting on hemoglobin to make methemoglobin

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11
Q

describe beta hemolysis

A

aka complete hemolysis
complete lysis of RBC in media around bacterial colonies and is result of hemolysin enzyme produced by specific bacterial species

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12
Q

describe gamma hemolysis

A

NOT hemolysis at all
absence of hemolysis surrounding non-hemolytic colonies

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13
Q

ID the types of hemolysis indicated by the arrows

A
  1. beta hemolysis
  2. alpha hemolysis
  3. gamma hemolysis
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14
Q

what is the most common organism isolated from cases of pyoderma in dogs/cats?

A

Staphylococcus pseudintermedius

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15
Q

Biopsies are the sample of choice for diagnosing bacterial dermatitis in what type of skin lesions?

A

Papules

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16
Q

When operating normally, our bacteriology course laboratory is conducted under which of the following biosafety levels?

A

BSL-1

17
Q

Which organisms may be found as part of the healthy skin microbiota?

A

a) Commensal organisms
b) Opportunistic organisms
c) Pathogenic organisms

18
Q

Which of the following forms of hemolysis is best described as a partial, greenish halo around bacterial colonies?

A

alpha

19
Q

The differential qualities of TSA+5% sheep blood agar allow it to discriminate bacteria that are what?

A

Hemolytic vs. non-hemolytic

20
Q

what type of microscope is useful for seeing internal details on unstained, living organism?

A

phase-contrast microscope

21
Q

what microscope type is used to generate highly magnified 3D image of bacteria?

A

scanning electron microscope

22
Q

Native or exotic agents that may be aerosolized and cause serious disease in humans, like Mycobacterium tuberculosis, are handled under which biosafety level?

A

BSL-3

23
Q

What factors are thought to affect the skin microbiota?

A

breed, diet, medications

24
Q

Which of the following best describes the TSA+5% sheep blood agar plates used in lab?

A

differential media

25
Q

Which of the following forms of hemolysis is best described as the complete lysis of red blood cells in the media around bacterial colonies?

A

beta

26
Q

which of the following stains highlights gram positive organisms?

A

crystal violet

27
Q

which of the following stains highlights gram negative organisms?

A

gram’s safranin

28
Q

Which of the following microscopes is the most commonly used in clinical medicine, and is the sort we use in our bacteriology laboratory?

A

bright field microscope

29
Q

which microscope is useful for visualizing extremely small or thin bacterial, like Borrelia?

A

dark field microscope

30
Q

At what biosafety level do most diagnostic laboratories operate at?

A

BSL-2

31
Q

What biosafety level would be required to handle Ebola virus?

A

BSL-4

32
Q

what biochemical tests is looking for an enzyme not commonly present in members of the family Enterobacteriaceae?

A

oxidase test

33
Q

what test can help to quickly discriminate Gram-negative organisms from Gram- positive ones?

A

KOH test

34
Q

what biochemical tests is looking for an enzyme which can reduce hydrogen peroxide to water and oxygen, with the appearance of bubbling in a positive test?

A

catalase test

35
Q

what test is looking for an enzyme which converts fibrinogen to fibrin?

A

coagulase test

36
Q

ID hemolysis type

A

beta

37
Q

ID hemolysis type

A

alpha

38
Q
A

Microbiology (29 decks)

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