Lecture 19 - Campylobacter + Bordetella Flashcards by Raegan Chambers

What are the two major characteristics based on the behavior of Campulobacter?

Commensal + Intracellular

How well did you know this?

Not at all

Perfectly

Where is Campylobacter found?

GI and Gential tracts

How well did you know this?

Not at all

Perfectly

What can an infection from Campylobacter cause?

Diarrhea + Abortion

How well did you know this?

Not at all

Perfectly

What is special about the morphology of Camplyobacter?

Gull-winged

Spiral

How well did you know this?

Not at all

Perfectly

Campylobacter: oxygen requirements

Micoaerophilic

How well did you know this?

Not at all

Perfectly

What are the % of oxygen and CO2 that Campylobacter like?

5 to 10% O2

1 to 10% CO2

How well did you know this?

Not at all

Perfectly

Campylobacter: oxidase

Positive

How well did you know this?

Not at all

Perfectly

Campylobacter: catalase

varies

How well did you know this?

Not at all

Perfectly

Campylobacter: MacConkey

Growth, w/ no fermentation (yellow)

How well did you know this?

Not at all

Perfectly

What are the three Campylobacter strains that are responsible for verneral disease?

C. Fetus
C. Veneralis
C. Jejuni

How well did you know this?

Not at all

Perfectly

Where do most Campylobacter outbreaks occur?

Western states

How well did you know this?

Not at all

Perfectly

Which strain of Campylobacter is responsible for FBI’s in poultry?

C. Jejuni

How well did you know this?

Not at all

Perfectly

What are the virulence factors that Camplyobacter contain?

Flagellar genes
Adhesins
Cytolehal distending toxin
S layer

How well did you know this?

Not at all

Perfectly

What is another name for the S layer?

Microcapsule

How well did you know this?

Not at all

Perfectly

What Campylobacter strains have an S layer?

C. Venerealis

C. Fetus

How well did you know this?

Not at all

Perfectly

What is the general structure of the S layer?

Lattice

How well did you know this?

Not at all

Perfectly

What does the S layer do?

Reduces susceptibility to pahgocytosis and complement

How well did you know this?

Not at all

Perfectly

What is important about the S layer when it comes to chronic infections?

Has 8 different variants, allows for switching to keep hidden from the immune system

How well did you know this?

Not at all

Perfectly

Which strains of Campylobacter has CDT?

C. Fetus

C. Jejuni

How well did you know this?

Not at all

Perfectly

What does CDT cause?

Cell-cycle arrest
Distended host cell morphology
Leading to apoptosis

How well did you know this?

Not at all

Perfectly

What does apoptosis via CDT allow for?

Releases bacteria within the cell

How well did you know this?

Not at all

Perfectly

Where is C. vernrealis commonly found?

Bovine reproductive tract

How well did you know this?

Not at all

Perfectly

What does C. venerealis cause?

Veneral disease in cattle
Early embryonic death
Infertility

How well did you know this?

Not at all

Perfectly

Where does C. fetus commonly infect?

Intestinal tract of sheep, goats, and cattle

How well did you know this?

Not at all

Perfectly

What does C. fetus tend to cause?

Abortion and stillbirths in sheep and goats Weak offspring Sporadic abortions in cattle

What does C. jejuni commonly infect?

Intestinal tract of birds and mammals

What does C. jejuni commonly cause in sheep?

Abortion

What does C. jejuni commonly cause in dogs?

Enteritis

What does C. jejuni commonly cause in birds?

Hepatitis

What disease process is caused by venerealis in cattle?

Bovine genital campylobacteriosis

What does Bovine genital campylobacteriosis cause in cattle?

Temporary infertility | Occasional abortion

What is the length on infection in bulls with C. venerealis?

Indefinite

Where does the infection spread in cattle in regards to C. venerealis?

uterus

How can C. Venerealis infect bulls indefinitely?

Antigenic SHIFT of S layer

What is the transmission route for C. Fetus?

Fecal - oral

What does C. fetus cause in Birds?

Hepatitis

What does C. fetus cause in cattle, pigs, and ewes?

Hemorrhagic enteritis + diarrhea

What causes Ovine genital campylobacteriosis?

C. Fetus + C. Jejuni

What are the symptoms of Ovine genital campylobacteriosis ?

Abortion | Necrotic placentitis

What is the abortion pattern in ewes with Ovine genital campylobacteriosis?

Cyclical every 4 to 5 years

Where does C. fetus localize in Ewes?

Uterus

What disease process does C. Jejuni cause in birds?

Avian vibrionic hepatitis

What are the symptoms of Avian vibrionic hepatitis?

Drop in egg production | Necrotic liver

What causes canine intestinal campylobacteriosis?

C. Jejuni

Whats a good way to note that Campylobacter may have been the cause of death in an aborted lamb?

Hepatic lesions

What are the treatment methods for C. Fetus?

Therapeutic vaccination Isolation Antibiotics

What is the AB used for C. Fetus?

Chlortetracycline

What are the treatment methods for C. Jejuni?

Antibiotics

What is the AB used for C. Jejuni?

Dihydrostreptomycin

What are the treatment methods for C. Venerealis?

Antibiotics

What is the AB used for C. Venerealis?

Dihydrostretomycin

Where is bordetella a commensal bacteria?

URT of mammals and birds

What does bordetella have an affinity for?

Ciliated respiratory epithelium

What are the three major bordetella species?

Bronchospetica Avium Parapertussis

Bordetella: oxygen requirements

Aerobic

Bordetella: Gram

negative

Bordetella: oxidase

positive

Bordetella: Catalase

postive

Bordetella: energy source

AA's

Bordetella: virulence factors

Adhesins | Toxins

What types of adhesins does bordetella have?

Pertactin | Fimbriae

What are the four toxins found in bordetella?

Tracheal cytotoxin Dermonecrotoxin toxin Osteotoxin LPS

What does the tracheal cytotoxin do?

Inhibits movement of cilia and tracheobronchial clearance | Can kill ciliated cells

What symptom does tracheal cytotoxin cause?

Coughing

What does dermonecrotoxic toxin cause?

Skin necrosis | Differentiation of osteoblasts

What symptom does dermonecrotoxin cause?

Turbinate atrophy

What does Osteotoxin cause?

Kills osteoblasts

What three virulence factors are seen in B. Bronchoseptica?

Filamentous hemagglutinin T3SS Adenylate cyclase-hemolysin

What animals are infected by B. parapertussis?

Lambs

What does B. parapertussis cause in lambs?

Pneumonia

B. Bronchispectica: Pigs

Atrophic rhinitis

B. Bronchispectica: Dogs

Canine infectious tracheobronchitis

B. Bronchispectica: Kittens

Pneumonia

B. Bronchispectica: Horses

Respiratory infection

B. Bronchispectica: rabbits

URI

B. Bronchispectica: Lab rodents

Bronchopneumonia

B. Avium: Turkeys

Coryza

What commmon bacteria is a co-infection with Atrophic rhinitis?

Pasturella

What are the symptoms of Kennel cough?

Coughing, retching, mild serous oculonasal discharge

What are the symptoms of turkey coryza?

Beak breathing - Sneezing - Excesive lacrimation

What is seen grossly in turkeys with B. Avium?

renal granulomas | flattened trachea

What secondary infection occurs commonly in birds with B. Avium?

E. coli

B. Bronchispectica: hemolysis

B. Bronchispectica: urease

B. avium + parapertussis: hemolysis

B. avium + parapertussis: urease