Lecture 16 - Salmonella Flashcards
What are the three major animal pathogens in the enterobacteriacea family?
Salmonella + E. Coli + Yersinia
What are the opportunistic animal pathogens in the enterobacteriacea family?
Proteus + Klebsiella + Enterobacter
What is the gram stain of the strains within Enterobacteriacea?
Negative
Is Enterobacteriacea catalase positive or negative?
Positive
Is Enterobacteriacea oxidase positive or negative?
Negative
What is the oxygen requirements of bacteria within the Enterobacteriacea family?
Facultative anaerobes
Which of the Enterobacteriacea ferment lactose?
E. coli + enterobacter + klebseilla
What are the types of IMViC tests?
indole + methyl red + voges-proskauer + citrate
What does the methyl red test show?
pH
What does the voges-proskauer show?
Fermentation
Which of the Enterobacteriacea are hemolytic (some at least)?
E. Coli
Which of the Enterobacteriacea have a swarming growth pattern on agar?
Proteus
Which of the Enterobacteriacea are NOT motile?
Klebsiella
Which of the Enterobacteriacea do all of them show + in the imdole test?
E. Coli
Which of the Enterobacteriacea have negative methyl red tests?
Enterobacter + Klebsiella
Which of the Enterobacteriacea show positive voges-proskauers in all strains?
Enterobacter + Klebsiella
Which of the Enterobacteriacea produce H2S?
Salmonella + Proteus
What are the two species of salmonella?
Enterica + Bongori
Where is Salmonella Bongori found?
Lizards
What are the three sub-types of Salmonella enterica that are important in vet medicine?
Typhimurium + Typhi + Enteriditis
What is the pattern of Salmonella in regards being a food borne pathogen?
Only zoonotic caused outbreak since 2007
What are most salmonella outbreaks linked to?
Poultry
How long can Salmonella live in the environment, if under the right conditions?
more then two years
Where does a lot of the food borne Salmonella come from, other then poultry?
Composted manure
Where does Salmonella tend to infect the most?
Intestines
What allows Salmonella to be eatten but still survive?
Acid tolerance response
acid shock proteins along with protecting in food and lipids
What cell type can kill Salmonella?
Neutrophils
What cell type can Salmonella grow in?
Macrophages
What does Salmonella do to attach to host cells?
Fimbrial attachement
via T3SS causes the host cell to remodel actin and take in the bacteria (cellular ruffling)
What does the salmonella do to the balance within the cell?
Disruption of ion balance
Inflammation + Fluid/ion balance disruption = diarrhea
What causes healthy adults to get salmonellosis?
STRESS
With dogs, horses, and cats what is important to note about salmonella even if it does not make them sick?
They can be asymptomatic carriers
What occurs with dogs and cats when they get Salmonellosis?
Acute diarrhea
+/- septicemia
When horses get Salmonellosis what is commonly seen?
Acute enteritis
+/- septicemia
What S. enterica types cause problems in horses?
Typhimurium
Enteriditis
Anatum
Newport
When is Salmonellosis seen in horses?
Post-surgery or Transport
Newborns
What are the three Salmonella types that affect cattle?
Dublin
Enteriditis
Typhimurium
What are the salmonella species that cause enterocolitis in cattle?
Enteriditis + Typhimurium
What does S. Dublin do in cattle, that is different then most presentations ?
Dry gangrene + Bone lesions in calves
Along with - osteomyelitis w/ spinal compression
Drop in milk production
What other symptoms do you see in cattle with S. Dublin?
Diarrhea + Fever + Depression + Abortion + Dehydration
What are the salmonella strains that affect poultry?
Pullorum
Gallinarum
Enteriditis
Arizonae
What are the three major diseases that Salmonella cause in poultry?
Fowl typhoid
Pullorum disease
Arizonsis
What Salmonella species causes Fowl typhoid?
S. Gallinarum
What does S. Pullorum do in poultry?
Infects ovaries then transmitted to the eggs
What does Pullorum disease look like?
Bacillary white diarrhea
Huddle in heat + anorexic + poor feathering + high mortality
When does Pullorum disease begin to show up in poultry?
What does S. Gallinarum do in poultry?
Infects ovaries then transmitted to the eggs
Causes fowl thyphoid
How does fowl thyphoid present in poultry?
Septiceamia in adults with sudden death
~ Pollurum in youngs birds
What does S. enteriditis do in poultry?
Infect ovaries and is transmitted to the eggs
What bird does S. Arizonae infect most?
Turkeys
What symptoms are seen with Arizonsis?
Death in young birds
Anorexia + Diarrhea + Paralysis + Blindness + Reduced egg production
What is the common resevoir for S. Arizonsis?
Snakes
What are the types of Salmonella that affect swine?
Choleraesuis
Enteriditis
Typhimurium
What are the Salmonella species that cause enteritis in swine?
Enteriditis + Typhmurium
What does S. Choleraesuis cause in swine?
Enterocolitis + Septicemia
Cyanosis is seen as well = systemic
What are the salmonella species that affect sheep?
Brandenburg
Dublin
Enteriditis
Typhimurium
What are the types of Salmonella that cause enteritis in sheep?
Enteriditis + Typhmurium
What does S. Dublin cause in sheep?
Enterocolitis + Septicemia
What does S. Brandenburg do in sheep?
Abortions
What kind of reporting is involved in Salmonella?
Foodborne Outbreak report tracing
What are the characteristics present within the Salmonella genera?
Motile Methyl red Citrate H2S producer Lysine Decarboxylase
What does Salmonella look like on an XLD plate?
Colonies with black within the center
What does a TSI test from Salmonella look like?
red slant
yellow butt
sulfide +
What does Salmonella look like on a MacConkey agar?
Growth, but no lactose fermentation
What can MacConkey help you differentiate Salmonella from?
E. Coli
How are companion animals with Salmonellosis treated?
FLUDS
Glucocorticoids to prevent shock
How are cattle and swine treated for Salmonellosis?
FLUIDS
Severe cases Antibiotics
How are poultry treated for Salmonellosis?
Antibiotics
What animals have a Salmonella vaccine?
Poultry + Cattle + Swine
What kind of vaccine type is the Cattle salmonella vaccine?
Bacterial extract + live inactivated
What kind of vaccine type is the Poultry salmonella vaccine?
Live inactivated + Modified live + Combos
What kind of vaccine type is the Swine salmonella vaccine?
Line inactivated