Lab Manual: Postnatal Blood Cell Development

Question 1

pluripotent stem cells in the bone marrow produce two kinds of stem cells:

Answer 1

myeloid and lymphoid stem cells.

Question 2

myeloid and lymphoid stem cells produce ____ cells, that are more limited in the kinds of cells they produce

Answer 2

progenitor cellss.

Question 3

myeloid stem cells produce ___ progenitors, ______ progenitors, and _____/____ progenitors.

Answer 3

produce 1. erthryocyte progenitors: makes RBCs via erythropoiesis 2. megakaryocyte progenitors: produce megakaryocytes during thrombopoiesis 3. granulocyte/monocyte progenitors, that produce granulocytes and monocytes during granulopoiesis and monopoieisis respectively.

Question 4

outline granulopoiesis

Answer 4

production of granulocytes. 1. granulocyte/monocyte progenitor cell 2. myeloblast 3. promyelocyte –> contains granules characteristic of each granulocyte it should become. 4. myelocyte (3 types) 5. metamyelocytes ( 3 types) 6. band (stab) cells (3 types) – U shaped/segmented nucleus. 7. mature granulocytes – neutrophils, basophils, or eosinophils

Question 5

outline monopoiesis

Answer 5

1. granulocyte/monocyte progenitor cell 2. monoblast 3. promonocute 4. mature monocyte during development, the cells become smaller and the nuclear indentation becomes more prominent.

Question 6

lymphocytes are produced by ____. outline this process

Answer 6

lymphopoiesis.

1. lymphoid stem cell
2. lymphoblast
3. prolymphocyte
4. lymphcyte

as the cell matures, it gets smaller.

Question 7

outline erythrocyte development

Answer 7

1. megakaryocyte/erythrocyte progenitor
2. erythrocyte progenitor
3. proerythroblast
4. basophilic erythroblast
5. polychromatophilic erythroblast
6. orthochromatophilic erythroblast (lymphopoiesis)
7. reticulocyte (now anucleated and appears similar to RBC except its cytoplasm contains a bluish network when strained).
8. erythrocyte.

as the cell matures, there is a loss of organelles (most noteably, the nucleus, and an increase in cytoplasmic hemoglobin)

Question 8

label

Answer 8

Question 9

outline thrombocyte developement

Answer 9

(platelet development)

1. megakaryocyte/erthryocyte progenitor
2. megakaryocyte progenitor
3. megakaryoblast (single unlobed nucleus)
4. megakaryocyte (single bilobed nucleus)
5. thrombocyte.