Kaplan Biochemistry: Chapter 6 DNA and Biotechnology

Question 1

DNA is ___ charged

Answer 1

neg

Question 2

Histones are __- charged

Answer 2

positive

Question 3

DNA is read ___ to ___ and synthesized ___ to ___

Answer 3

DNA is read 3’ to 5’ and synthesized 5’ to 3’

Question 4

the 3’ to 5’ strand is aka ____ or ____ strand

Answer 4

template or non coding strand.

Question 5

the 5’ to 3’ strand is aka ____ or ___ strand

Answer 5

5’ to 3’ strand is the coding strand or SENSE strand

Question 6

phosphodiester bonds links the 3’ ___ of one sugar to the 5’ ____ group

Answer 6

phosphodiester bonds links the 3’ carbon of one sugar to the 5’ phosphate group

Question 7

____ unwinds DNA, and ____ prevents supercoiling

Answer 7

HELICASE unwinds DNA, and TOPOISOMERASE prevents supercoiling

Question 8

____ places an RNA primer complementary to __ to ___ template strand

Answer 8

PRIMASE places an RNA primer complementary to 3’ to 5’ template strand

Question 9

________ EXTENDS AND ADDS NEW NUCLEOTIDES IN A ___ TO ___ DIRECTION, COMPLEMENTARY TO TEMPLATE STRAND, WHICH IS ___ TO ___

Answer 9

DNA POLYMERASE 3 EXTENDS AND ADDS NEW NUCLEOTIDES IN A 5’ TO 3’ DIRECTION, COMPLEMENTARY TO TEMPLATE STRAND, WHICH IS 3’ TO 5’

Question 10

RNA primers are removed and replaced with DNA by ______

Answer 10

DNA POLYMERASE I

Question 11

DNA gets sealed by _____

Answer 11

DNA LIGASE

Question 12

Role of DNA polymerase II

Answer 12

it has proofreading abilities. involved in damage repair caused by chemical agents.

Question 13

____ is compact DNA that is transcriptionally silent

Answer 13

heterochromatin

Question 14

____ loosened DNA that is genetically active

Answer 14

EUCHROMATIN.