HM MAN ch 19: clinical Laboratory Flashcards
what is the SF 545?
laboratory report display
when performing a capillary finger puncture make a quick stab no greater than how deep on the side of the finger?
2mm
when performing venipuncture apply the tourniquiet areound the arm approx how many inches above the intended site?
3 to 4 inches
when performing a venipuncture the HM must cleanse the desired site with what?
70% alcohol pad or
povidone-iodine
when performing a venipuncture what degree angle should the needle be introduced?
15-30 degrees
how long can a tourniquet be applied when performing a venipuncture?
no more than one minutes
___ is the stucture tah supports the magnification and focusing system of the microscope.
arm
___ is the handle by which the microscope is carried
arm
___ is the platform on which a specimen is placed for examination
stage
___ is composed of a compact lens system and is located below the stage.it concentrates and focuses light from the light source on the specimen
condenser
___ is located on the condenser to control the amount of light and angle of light rays.
iris diaphragm
how much magnification is provided by the low-power objective?
10x
how much magnification is provided by the high-power objective?
40x
how much magnification is provided by the oil immerision ovjective?
100x
the oil immersion objective is used for detailed study of what?
stained blood and bacterial smears
A complete blood count consists of what five tests?
- total RBC count
- Hemoglobin determination (Hgb)
- Hematocrit calculation (Hct)
- Total WBC count
- WBC differential count
A RBC count that drops below normal values may indicate what?
anemia
What are the normal values for hemoglobin in woman?
12 to 16
what are the normal values for hemoglobin in men?
14 to 18
packed RBC volume is also know as what?
hematocrit
what is hematrocrit?
the ratio of the volume of RBCs to the volume of whole blood
normally expressed as a percentage
what is the percentage of hematocrit in women?
37-47%
what is the percentage of hematocrit in men?
42-52%
what is the name for WBCs?
leukocycte
what should never be used to clean lenses on a microscope?
alcohol or similiar solvents
Normal WBC values in adults range from ___ to ___ cells per cubic millimeter.
4,800- 10,800
how are normal WBC values normally reported?
4.8-10.8 X 10 (upper case 6)/ml (per cubic millimeter)
When WBCs rise above normal values the condition is referred to as what?
leukocytosis
_____frequently occurs when systemic or local infections are present.
leukocytosis
When does leukocytosis frequently occur?
systemic or local infections are present
what are seven examples of other physiological conditions that can cause leukocytosis, other than systemic or local infections?
shortly after the mother gives birth pregnancy appendicitis ulcers emotional stress anxiety strenuous exercise
an abnormally low WBC count is known as what?
leukopenia
what is leukopenia?
low WBC count
Severe or advanced bacterial infections such as typhoid, partyphoid and sometimes tularemia can cause which WBC condition?
leukopenia
Measles, rubella, smallpox, hepatits, dengue fever and influenza can cause what WBC conditions
leukopenia
anaphylactic shock can cause what WBC conditions?
leukopenia
radiation can cause what WBC condition?
leudopienia
what are the five types of WBCs?
neutophils eosinophils basophils lymphocyctes monocyctes
Which WBC accounts for the largest percentage of leukocytes found in a normal blood sample?
neutorphils
Which WBC has the function to destroy parasites and respond in immediate allergic reactions?
Eosinophils
What is the most common cause of increased eosinophils worldwide?
parasites, in particular helminthic
a rise in this WBC is associated with inflammatory disorders and certain leukemias.
Basophil
The function of this WBC is associated with immune response and the body’s defense against viral infections
lyphocyte
what are the larges of the normal WBCs?
monocycte
what are the four growth requirements for bacteria?
temperature
oxygen
nutrition
moisture
what temperature do psychrophilic bacteria enjoy?
cold
4 degrees C
what are the three categories of bacteria temperature requirements?
Psychrophilic
mesophilic
thermophilic
What temperature do mesophilic bacteria best reproduce at?
body temp
35 degrees C
what temp do thermophilic bacteria reproduce at?
how
42 degrees C or higher
120 degrees F
what are the two types of bacteria oxygen requirement categories?
aerobes
anaerobes
___ are oganisms that reproduce in the presence of oxygen
aerobes
sounds like air
___ are organisms that do not reproduce in the presence of oxygen
anaerobes
what are the three distinct shapes or categories of bacteria?
Coccus
Bacillus
Spirochetes
what shape are coccus bacteria?
spherical
What shape are bacillus bacteria?
rod-shaped
what shape are spirochete bacteria?
spiral or corkscrew shaped
what are the two categories of toxins?
endotoxin
exotoxin
which type of toxin are highly poisonous and associated iwth septic shock?
exotoxin
which toxin is less potent but may affect the PT during the course of antibiotic therapy?
endotoxin
what color are gram stain positive cells?
crystal violet
what color are gram stain negative cells?
pink
what shape are gram negative cells?
rods
what shape are gram postive cells?
cocci
what shape and gram stain are streptococcus pneumoniae (pneumonia)?
cocci
gram stain positive
what shape and gram stain are streptococcus pyogenes (strep throat)?
cocci
gram stain positive
what shape and gram stain are neisseria gonorrhoeae (gonorrhea)?
cocci
gram stain negative
what shape and gram stain are neisseria meningitis?
cocci
gram stain negative
what shape and gram stain are corynebacterium diphtheriae?
Bacilli
gram stain positive
what shape and gram stain are botulism, tetanus and gas gangrene?
bacilli
gram stain positive
what shape and gram stain are yersinia pestis (bubonic plague)?
bacilli
gram stain negative
what shape and gram stain are bordetella pertussis (whooping cough)?
bacilli
gram stain negative
what does RPR stand for?
rapid plasma reagin
what does RPR test for?
syhilis
what are the two outcomes of available from a RPR test?
reactive or non-reactive
the ___ test is an accurate, 2 minute disposable test designed to detect the presence of infectious mononucleosis.
monospot test
how long does the monospot test take?
2 minutes
what is the simple and frequently used method of detecting fungi?
the potassium hydrodxide preparation KOH
to detect fungi in infected tissue , place skin, hair, or nail scrapingfrom the affected area on a glass slide and add one drop of _____.
10% KOH
which UA is the ideal screening specimen and why?
first morning urine specimen
usually concentrated and more likely to rebeal abnormalities
what are the normal adult urine volume ranges?
600-2,000 ml
the average is 1,500ml
What is the definition of urine specific gravity?
the density of a solution compared to an equal volume of distilled water
red or red-brown urine is caused by what?
blood
what causes yellow or brown urine?
bile
what causes olive green to brown-black urine?
phenols
what causes urine to be dark orange in color?
pyridium
topical analgesic used in the treatment of urinary tract infections
what are the normal specific gravity ranges during a 24 hour period?
1.015 to 1.030
Microscopic examination of urine sediment is usually performed at what level of magnification?
40X
high-powered
during a urine sediment exam there are normally ___ leukocytes per high power field.
0 to 3
more than ___ leukoyctes cells per high power field may indicate disease somewhere in the urinary tract.
3
How are Casts in urinary sediments formed?
coagulation of albuminous material in the kidney tubles
if cast are present in large numbers, the urine is almost sure to be positive for what?
albumin
What are four critical laboratory results?
- WBC count above 50,000 indicates an acute infection
- Heoglobin concentration below 7 indicates sever anemia and may require a transfustion
- Glucose and ketones in urine
- bacteria present in a gram stain from direct patient smears
A minimum file of what percent of certain ship’s company is required to be enrolled in the walking blood bank?
10%
What does a WBC count above 50,000 indicate?
the presence of an acute infection
what does hemoglobin concentrations below 7 indicate?
severe anemia that may require a transfusion
What does glucose and ketones in urine indicate?
uncontrolled diabetes