histology in medicine

Question 1

what is histology ?

Answer 1

microscopic analysis of tissues and organs in the body

Question 2

what type of microscope is histology primary use ?

Answer 2

light microscope

Question 3

what steps once the cells have been harvested in histology does the tissues undergo ?

Answer 3

-fixation
-embedding
-sectioning
-staining

Question 4

why do we study histology ?

Answer 4

intimate knowledge of the microscopic structure of cells , tissues and organs

understand what abnormal cells , tissue and organs look like

Question 5

what image does light microscope produce ?

Answer 5

can see cell but not internal structures

Question 6

what image does transmission electron microscope produce ?

Answer 6

can see internal structures

Question 7

what image does a scanning electron microscope produce ?

Answer 7

a 3d image

Question 8

why does tissue preparation require staining ?

Answer 8

provides contrast / colour to specimens

allows us to identify tissue structures

Question 9

what is the most common stain to use in order to prepare the tissue ?

Answer 9

haematoxylin and eosin (H&E)

Question 10

Is haematoxylin positively or negatively charged ? what type of molecule does this stain dye ?

Answer 10

positively charged

will stain negatively charged molecules (blue )

stains DNA

Question 11

Is eosin positively or negatively charged ? what type of molecule does this stain dye ?

Answer 11

negatively charged

stain positively charged molecules ( pink / red)

stain proteins

Question 12

is haematoxylin basic or acidic ?

Answer 12

basic

Question 13

is eosin acidic or basic ?

Answer 13

acidic

Question 14

what are the four types of tissues ?

Answer 14

epithelial
connective
muscle
nervous