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biochem > (F) Laboratory Experiments > Flashcards

(F) Laboratory Experiments Flashcards

1
Q

Carb Chem

Molisch test detects the presence of?

A

Carbohydrates

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2
Q

Carb Chem

Molisch Reagent

A. H2SO4
B. HCl
C. NaOH

A

A

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3
Q

Carb Chem

Positive indicator of Molisch test

A

Purple ring

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4
Q

T or F. Molisch Test only detects the presence of polysacharrides.

A

F (all carbs - mono, di, poly)

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5
Q

Carb Chem

Bial test detects the presence of?

A

Pentose Sugars (ex: Ribose)

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6
Q

Carb Chem

Positive indicator of Bial

A

Blue-green

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7
Q

Carb Chem

Bial’s reagent

A. FeCl3
B. HCl
C. Orcinol
D. All of the above

A

D

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8
Q

Carb Chem

Moore detects the presence? which forms a positive indicator of?

A
  • Reducing sugar
  • Yellow to Brown
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9
Q

Carb Chem

Moore’s Reagent

A. H2SO4
B. HCl
C. NaOH

A

C (30%)

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10
Q

T or F. Seliwanoff detects the presence of galactose, glucose, glyceraldehyde, fructose, ribose, and xylose.

A

F (those are reducing sugars that can be detected by Moore’s, Benedict’s, and Fehlings ; Seliwannof is a test for ketose )

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11
Q

Carb Chem

Seliwanoff detects the presence of? which creates a positive indicator of?

A

ketohexoses
red color

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12
Q

T or F. The Seliwanoff test can detect the presence of ketoses and aldoses (in a longer contact time)

A

T

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13
Q

T or F. Seliwanoff test positive for Galactose and Glucose.

A

F (Fructose and Sucrose)

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14
Q

Carb Chem

Iodine detects the presence of? which forms a positive indicator of?

A

starch and polysaccharide

Amylase - blue or black
Dextrin - black
Glycogen - reddish brown

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15
Q

Carb Chem

Benedict’s positive results

A

reddish brown

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16
Q

Carb Chem

Benedict’s reagents
A. Sulferic citrate
B. Sodium Potassium
C. Pentahydrate of copper sulfate
D. Resorcinol

A

C (also sodium citrate/carbonate)

17
Q

T or F. Benedicts detect the presence as same as Moore & Fehling’s test.

A

T (reducing sugars)

18
Q

Carb Chem

Fehlings detect the presence of? which forms a positive indicator of?

A

Reducing
Yellow or Brown (according to mam adlene’s carb lecture brick-red din same as benedict’s)

19
Q

Carb Chem

T or F. Moore, Benedicts and Fehlings test positive in Sucrose and Ribose.

A

F (sucrose is NOT a reducing sugar)

20
Q

Carb Chem

T or F. Iodine has a negative pre-hydrolysis and a positive post hydrolysis

A

F (opposite)

21
Q

Carb Chem

Identify the pre- and post hydrolysis of Benedicts

A

Negative (pre) & Positive red (post)

22
Q

Benedict’s Test in urine

Positive indicator

A

Red (2%)

23
Q

Benedict’s Test in urine

color of 0.5 sugar

A

green (lowest)

24
Q

Benedict’s Test in urine

T or F. Orange is 2%.

A

F (1.5)

25
Q

Benedict’s Test in urine

1% of sugar

A

Yellow

26
Q

Protein Digestion

responsible to convert proteins into proteoses and peptones

A

Pepsin

27
Q

Protein Digestion

an enzyme that aids w/ digestion

A

trypsin

28
Q

Protein Digestion

found in small intestine

A

trypsin

29
Q

Protein Digestion

What will hydrolyze protein bonds into simpler peptide bonds?

A

Acetic acid

30
Q

Protein Digestion

Considered as “complete protein”

A

MELK

31
Q

Protein Digestion

Positive result of Biuret test

A

Pink and Violet

32
Q

Protein Digestion

positive pink result indicates

A

shorter polypeptide chain

33
Q

Protein Digestion

positive violet result indicates

A

longer peptide chain

34
Q

Protein Digestion

Negative result of Biuret test

A

Blue

35
Q

Protein Digestion

a negative blue result indicates

A

lack of protein duh or the presence of its building blocks which is aa duh

biochem (31 decks)

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