(F) Laboratory Experiments

Question 1

Carb Chem

Molisch test detects the presence of?

Answer 1

Carbohydrates

Question 2

Carb Chem

Molisch Reagent

A. H2SO4
B. HCl
C. NaOH

Answer 2

A

Question 3

Carb Chem

Positive indicator of Molisch test

Answer 3

Purple ring

Question 4

T or F. Molisch Test only detects the presence of polysacharrides.

Answer 4

F (all carbs - mono, di, poly)

Question 5

Carb Chem

Bial test detects the presence of?

Answer 5

Pentose Sugars (ex: Ribose)

Question 6

Carb Chem

Positive indicator of Bial

Answer 6

Blue-green

Question 7

Carb Chem

Bial’s reagent

A. FeCl3
B. HCl
C. Orcinol
D. All of the above

Answer 7

D

Question 8

Carb Chem

Moore detects the presence? which forms a positive indicator of?

Answer 8

• Reducing sugar
• Yellow to Brown

Question 9

Carb Chem

Moore’s Reagent

A. H2SO4
B. HCl
C. NaOH

Answer 9

C (30%)

Question 10

T or F. Seliwanoff detects the presence of galactose, glucose, glyceraldehyde, fructose, ribose, and xylose.

Answer 10

F (those are reducing sugars that can be detected by Moore’s, Benedict’s, and Fehlings ; Seliwannof is a test for ketose )

Question 11

Carb Chem

Seliwanoff detects the presence of? which creates a positive indicator of?

Answer 11

ketohexoses
red color

Question 12

T or F. The Seliwanoff test can detect the presence of ketoses and aldoses (in a longer contact time)

Answer 12

T

Question 13

T or F. Seliwanoff test positive for Galactose and Glucose.

Answer 13

F (Fructose and Sucrose)

Question 14

Carb Chem

Iodine detects the presence of? which forms a positive indicator of?

Answer 14

starch and polysaccharide

Amylase - blue or black
Dextrin - black
Glycogen - reddish brown

Question 15

Carb Chem

Benedict’s positive results

Answer 15

reddish brown

Question 16

Carb Chem

Benedict’s reagents
A. Sulferic citrate
B. Sodium Potassium
C. Pentahydrate of copper sulfate
D. Resorcinol

Answer 16

C (also sodium citrate/carbonate)

Question 17

T or F. Benedicts detect the presence as same as Moore & Fehling’s test.

Answer 17

T (reducing sugars)

Question 18

Carb Chem

Fehlings detect the presence of? which forms a positive indicator of?

Answer 18

Reducing
Yellow or Brown (according to mam adlene’s carb lecture brick-red din same as benedict’s)

Question 19

Carb Chem

T or F. Moore, Benedicts and Fehlings test positive in Sucrose and Ribose.

Answer 19

F (sucrose is NOT a reducing sugar)

Question 20

Carb Chem

T or F. Iodine has a negative pre-hydrolysis and a positive post hydrolysis

Answer 20

F (opposite)

Question 21

Carb Chem

Identify the pre- and post hydrolysis of Benedicts

Answer 21

Negative (pre) & Positive red (post)

Question 22

Benedict’s Test in urine

Positive indicator

Answer 22

Red (2%)

Question 23

Benedict’s Test in urine

color of 0.5 sugar

Answer 23

green (lowest)

Question 24

Benedict’s Test in urine

T or F. Orange is 2%.

Answer 24

F (1.5)

Question 25

# Benedict's Test in urine 1% of sugar

Answer 25

Yellow

Question 26

# Protein Digestion responsible to convert proteins into proteoses and peptones

Answer 26

Pepsin

Question 27

# Protein Digestion an enzyme that aids w/ digestion

Answer 27

trypsin

Question 28

# Protein Digestion found in small intestine

Answer 28

trypsin

Question 29

# Protein Digestion What will hydrolyze protein bonds into simpler peptide bonds?

Answer 29

Acetic acid

Question 30

# Protein Digestion Considered as "complete protein"

Answer 30

MELK

Question 31

# Protein Digestion Positive result of Biuret test

Answer 31

Pink and Violet

Question 32

# Protein Digestion positive pink result indicates

Answer 32

shorter polypeptide chain

Question 33

# Protein Digestion positive violet result indicates

Answer 33

longer peptide chain

Question 34

# Protein Digestion Negative result of Biuret test

Answer 34

Blue

Question 35

# Protein Digestion a negative blue result indicates

Answer 35

lack of protein duh or the presence of its building blocks which is aa duh